Methods and applications of in vivo CRISPR screening

Area of Science:

• Genetics
• Genomics
• Systems Biology

Background:

• Understanding genotype-phenotype connections is crucial for health and disease research.
• Pooled genetic screens offer unbiased, large-scale genotype-phenotype mapping.
• In vivo application of pooled CRISPR-Cas screens is emerging for broader gene function discovery.

Purpose of the Study:

• To review essential criteria for designing and implementing in vivo pooled CRISPR-Cas screening experiments.
• To focus on pooled CRISPR-based screens in mice.
• To highlight the potential of these screens combined with multi-omics and AI for scientific advancement.

Main Methods:

• Review of methodologies for in vivo pooled CRISPR-Cas screening.
• Focus on experimental design and implementation in mouse models.
• Integration of multi-omics data and artificial intelligence.

Main Results:

• Provides a framework for understanding and executing in vivo CRISPR screens.
• Identifies key considerations for successful pooled screening in mice.
• Emphasizes the synergistic potential of screening data with advanced analytical techniques.

Conclusions:

• In vivo pooled CRISPR-Cas screens are powerful tools for accelerating gene function discovery.
• Careful design and implementation are critical for maximizing the utility of these screens.
• The integration of resulting datasets with multi-omics and AI promises fundamental breakthroughs in basic and translational science.