Related Experiment Video
Updated: Sep 13, 2025

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses
Published on: April 4, 2019
Genomic characterization and phylogenetic analysis of respiratory syncytial virus F gene in Sri Lanka: a comparative
Thejanee Perera1, Ishani De Silva2, Pavithri Bandara3
1Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Colombo, 00300, Sri Lanka.
Background:
Respiratory Syncytial Virus (RSV) is a major cause of acute respiratory tract infections (ARTI) worldwide, with seasonal outbreaks often influenced by climatic factors. This study investigates the genetic diversity and phylogenetic relationships of RSV strains circulated in Sri Lanka between 2022 December and 2024 February.
Results:
Subtyping of 12 RSV-positive samples identified both RSV A and B, with full-length F gene sequences obtained for four samples. Phylogenetic analysis revealed clustering with globally circulating strains. Mutation profiling highlighted key amino acid substitutions, including a rare mutation of N116D in the F protein, which may have functional implications. The limited sample size necessitates further studies with broader geographic coverage for more robust conclusions.
Conclusion:
This study provides insights into the genetic diversity of RSV F gene in Sri Lanka.
More Related Videos
09:08Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus
Published on: July 27, 2021
09:01An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection
Published on: December 10, 2013
Related Concept Videos
Rous Sarcoma Virus (RSV) and Cancer
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
Single Nucleotide Polymorphisms-SNPs