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Updated: Sep 13, 2025

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants

Published on: May 5, 2023

Endophytic Diversity in Sicilian Olive Trees: Identifying Optimal Conditions for a Functional Microbial Collection.

Dalila Crucitti^1,2, Stefano Barone², Salvadora Navarro-Torre³

¹Institute of Biosciences and Bioresources (IBBR), National Research Council (CNR), Via Ugo La Malfa 153, 90146 Palermo, Italy.

|July 30, 2025

Summary

Wild olive trees and winter sampling yielded diverse endophytic microbes with plant-growth-promoting traits. Organic farming enhanced microbial richness, supporting sustainable olive cultivation through bio-inoculants.

Keywords:

Olea europaea L. subsp. europaea var. europaea Olea europaea L. subsp. europaea var. sylvestris antifungal activity cv. ‘Nocellara Etnea’cv. ‘Nocellara Messinese’cv. ‘Nocellara del Belice’olive microbiota plant growth-promoting microorganisms

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Area of Science:

Microbiology
Plant Science
Agronomy

Background:

Sustainable agriculture requires understanding plant-microbe interactions.
Endophytic microorganisms offer potential for enhancing crop health and productivity.
Olive (Olea europaea L.) cultivation can benefit from microbial bio-inoculants.

Purpose of the Study:

To identify optimal conditions for isolating culturable endophytic microorganisms from olive trees.
To characterize the taxonomic diversity and plant-growth-promoting (PGP) traits of olive endophytes.
To explore the potential of these endophytes as bio-inoculants for sustainable olive cultivation.

Main Methods:

Culture-dependent isolation of endophytes from leaves and twigs of cultivated and wild olive trees.
Analysis of endophyte taxonomic diversity across bacterial and fungal phyla.
Screening of isolates for plant-growth-promoting (PGP) traits, including indole-3-acetic acid production, nitrogen fixation, and antifungal activity.

Main Results:

133 endophytic isolates were identified, with wild olive trees and twigs yielding greater diversity.
Winter sampling enriched the collection's taxonomic genera.
Organic farming systems supported higher microbial richness and diversity compared to conventional systems.
Bacterial isolates, particularly Bacillus spp., exhibited significant PGP traits and antifungal activity against Neofusicoccum vitifusiforme.

Conclusions:

Mediterranean olive endophytes possess significant diversity and functional potential.
Native microbial communities can serve as effective bio-inoculants for improving olive growth, nutrient uptake, and disease resistance.
This research provides a foundation for developing targeted microbial consortia for olive biocontrol and stress tolerance.