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Related Experiment Video

Updated: Sep 13, 2025

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Paeoniflorin Improves Stroke by Modulating the ESR1 Pathway: Data Mining and Validation Based on Network Approaches.

Zhenshan Sun1,2, Junjie Peng3,4, Jiangbangrui Chu3

  • 1Department of Biology, Faculty of Science, Hong Kong Baptist University, Hong Kong SAR 999077, China.

Pharmaceuticals (Basel, Switzerland)
|July 30, 2025
PubMed
Summary

This study identified seven key Traditional Chinese herbs for stroke treatment. Paeoniflorin (PF) was found to protect neurons by increasing Estrogen Receptor 1 (ESR1) expression under ischemic conditions.

Keywords:
Data-Mining-Derived ApproachEstrogen Receptor 1network pharmacologypaeoniflorinstroketraditional Chinese medicine

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Area of Science:

  • Integrative Medicine
  • Pharmacology
  • Neuroscience

Background:

  • Traditional Chinese Medicine (TCM) offers therapeutic benefits for stroke, but clinical data is fragmented.
  • Translational research faces challenges due to the dispersed nature of TCM clinical cases.
  • A novel approach is needed to systematically analyze TCM treatment patterns and active compound interactions for stroke.

Purpose of the Study:

  • To identify core Traditional Chinese herbs and active compounds for stroke treatment.
  • To elucidate the interaction mechanisms between active compounds and stroke-related targets.
  • To experimentally validate the therapeutic potential of identified compounds in a stroke model.

Main Methods:

  • Bioinformatics analysis of stroke-related targets from public databases (GEO, DisGeNET, Genecards).
  • Extraction of active ingredients using BATMAN-TCM 2.0 and network construction with Cytoscape.
  • Molecular docking using Autodock to predict compound-protein interactions.
  • In vitro experimental validation using HT22 cells subjected to oxygen-glucose deprivation-reperfusion (OGD/R).

Main Results:

  • Seven core herbs for stroke treatment were identified: Angelicae Sinensis Radix, Pheretima, Chuanxiong Rhizoma, Persicae Semen, Astragali Radix, Carthami Flos, and Radix Paeoniae Rubra.
  • Paeoniflorin (PF) was identified as a key active compound, with its interaction with Estrogen Receptor 1 (ESR1) highlighted.
  • In vitro studies confirmed that PF inhibits apoptosis and increases ESR1 expression in OGD/R-induced neuronal injury.

Conclusions:

  • Seven key Traditional Chinese herbs for stroke were identified through a systematic approach.
  • Paeoniflorin (PF) shows significant potential as a novel therapeutic compound for stroke.
  • Experimental validation supports PF's neuroprotective effects via ESR1 modulation under ischemic conditions.