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Increasing the Sensitivity of Aspergillus Galactomannan ELISA Using Silver Nanoparticle-Based Surface-Enhanced Raman

A D Vasilyeva1, L V Yurina1, E G Evtushenko1,2

  • 1N. M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, 4 Kosygina Str., 119334 Moscow, Russia.

Sensors (Basel, Switzerland)
|July 30, 2025
PubMed
Summary
This summary is machine-generated.

This study presents a highly sensitive method for detecting galactomannan (GM), a biomarker for invasive aspergillosis. The new technique significantly improves detection limits in biological fluids, aiding early diagnosis in immunocompromised patients.

Keywords:
2,3-diaminophenazineELISAaspergillosisgalactomannano-phenylenediaminesurface-enhanced Raman scattering

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Area of Science:

  • Biochemistry
  • Medical Diagnostics
  • Nanotechnology

Background:

  • Galactomannan (GM) is a key biomarker for invasive aspergillosis, a serious fungal infection.
  • Current diagnostic methods for GM have limitations in sensitivity and detection limits.
  • Surface-enhanced Raman scattering (SERS) offers superior detection capabilities for enzymatic reactions.

Purpose of the Study:

  • To develop a highly sensitive enzyme-linked immunosorbent assay (ELISA) for galactomannan (GM) quantification.
  • To apply a surface-enhanced Raman scattering (SERS)-based horseradish peroxidase (HRP) detection protocol to ELISA for enhanced GM measurement.
  • To improve the diagnostic sensitivity for invasive aspergillosis through precise GM quantification.

Main Methods:

  • Adaptation of a sensitive SERS-based HRP measurement protocol for ELISA.
  • Quantification of galactomannan (GM) in biological fluid samples.
  • Comparison of SERS-ELISA sensitivity against traditional colorimetric ELISA methods.

Main Results:

  • Achieved a detection limit of 4.3 pg per sample for GM.
  • Demonstrated a detection limit one and a half orders of magnitude lower than colorimetric detection using o-phenylenediamine.
  • Showcased fivefold higher sensitivity compared to standard ELISA using 3,3',5,5'-tetramethylbenzidine.

Conclusions:

  • The SERS-based ELISA protocol provides a highly sensitive and accurate method for GM quantification.
  • This enhanced diagnostic approach offers significant improvements over existing methods for detecting invasive aspergillosis.
  • The improved sensitivity facilitates earlier and more reliable diagnosis of invasive aspergillosis in at-risk patients.