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Updated: Sep 13, 2025
08:38
Protein Membrane Overlay Assay: A Protocol to Test Interaction Between Soluble and Insoluble Proteins in vitro
Published on: August 14, 2011
22.2K
Complete Sequence Analysis of Grapevine Leafroll-Associated Virus 4 and Interactions Between the Encoded Proteins
Tingting Du1, Yuxin Hao1, Jie Gao1
1National Center for Eliminating Viruses from Deciduous Fruit Tree, Research Institute of Pomology of CAAS, Xingcheng 125100, China.
Viruses
|July 30, 2025
Summary
Grapevine leafroll disease is a major threat to vineyards. Researchers sequenced two Chinese Grapevine leafroll-associated virus 4 (GLRaV-4) genomes, revealing distinct groupings and protein interactions that may drive viral pathogenicity.
Area of Science:
- Plant virology
- Molecular biology
- Genomics
Background:
- Grapevine leafroll disease poses a significant threat to global viticulture.
- Grapevine leafroll-associated virus 4 (GLRaV-4) is a primary causative agent of this disease.
Purpose of the Study:
- To obtain and analyze complete genome sequences of two Chinese GLRaV-4 isolates.
- To investigate the phylogenetic relationships of these isolates.
- To explore protein-protein interactions within GLRaV-4.
Main Methods:
- High-throughput sequencing
- Overlapping RT-PCR and RACE technology
- Phylogenetic analysis
- Yeast two-hybrid assays
- Bimolecular fluorescence complementation
Main Results:
- Complete genome sequences of two Chinese GLRaV-4 isolates were determined.
- Phylogenetic analysis placed these isolates into distinct branches within GLRaV-4 Group 1.
- Significant interactions were identified between GLRaV-4-encoded p23, HSP70, and CP proteins.
Conclusions:
- This study provides the first complete genome sequences for two distinct GLRaV-4 isolates from China.
- The p23 protein's interactions suggest a crucial role in GLRaV-4 pathogenicity.
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