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Comparative Study of Two Immunisation Protocols in Goats Using Thiol-Sepharose Chromatography-Enriched Extracts from
Magnolia M Conde-Felipe1,2, José Adrián Molina1, Antonio Ruiz1
1Parasitology Unit, Department of Animal Pathology, Faculty of Veterinary Medicine, University of Las Palmas de Gran Canaria, 35413 Gran Canaria, Spain.
A simplified vaccine protocol using less protein extract from Haemonchus contortus worms and fewer inoculations effectively reduced parasite burden and fecal egg counts in sheep. This offers a promising approach for controlling this parasitic infection in field conditions.
Area of Science:
- Veterinary Parasitology
- Immunology
- Animal Health
Background:
- Comparative analysis of two immunisation protocols for Haemonchus contortus infection.
- Protocols used different protein extract amounts (625 μg vs. 200 μg) and inoculation numbers (five vs. two).
Purpose of the Study:
- To evaluate the immunoprotective efficacy of simplified vaccination protocols against Haemonchus contortus.
- To assess the impact of reduced immunogen dose and inoculation frequency on parasite control.
Main Methods:
- Animals were immunised, challenged with H. contortus larvae (L3), and monitored for 8 weeks post-infection.
- Parasitological (worm burden, fecal egg count), biopathological (hematocrit), and serological (IgG, IgA) parameters were assessed.
Main Results:
- Both protocols achieved similar reductions in fecal egg counts (60.5-64.9%) and total worm burden (47.5-50%).
- Immunised groups showed early haematocrit recovery and increased H. contortus-specific IgG and IgA antibody levels.
Conclusions:
- A simplified protocol with lower immunogen doses and fewer inoculations can induce protective responses against H. contortus.
- This approach mitigates parasite pathology and reduces disease transmission, showing potential for field application.
