PsoRPM3 Recognises the Meloidogyne incognita Effector MiTSPc to Trigger Defence Response in Prunus sogdiana

Phytoparasitic nematodes are among the most economically destructive plant pathogens. Large numbers of effectors secreted by phytoparasitic nematodes are delivered into host cells to facilitate susceptible invasion and maintain long-lasting parasitism in the host plants. Plant nucleotide-bound leucine-rich repeat (LRR) receptors (NLRs) directly or indirectly recognise pathogen-derived effectors to initiate innate immunity. In this study, we have identified Meloidogyne incognita secreted effectors MiTSPc and MiACPS, which can interact with resistance protein PsoRPM3 in Prunus sogdiana (P. sogdiana). In the leaves of PsoRPM3 transgenic tobacco plants and disease-resistant P. sogdiana lines, when the MiTSPc and MiACPS were transit expressed, significant hypersensitive response and high ion leakage rate were detected. Moreover, when the MiTSPc was silenced in M. incognita, galls were observed in the roots of PsoRPM3 transgenic tobacco plants. Co-localisation experiments have shown that MiTSPc and PsoRPM3 were overlapped. Our data revealed that LxxLxLxxN/CxL motif of PsoRPM3 LRR domain can recognise MiTSPc^23-54aa. Taken together, the disease resistant protein PsoRPM3 can directly recognise M. incognita effector MiTSPc to deploy defence responses in P. sogdiana.