Silicon-Based Agent Attenuates Age-Related Spermatogenesis Disorder

Area of Science:

• Reproductive biology
• Gerontology
• Biochemistry

Background:

• Declining birth rates are linked to delayed marriage and age-related decline in male fertility.
• Spermatogenetic function naturally decreases with age, impacting reproductive capabilities.

Purpose of the Study:

• To investigate the efficacy of a silicon-based (Si-based) agent generating hydrogen in improving spermatogenic function in aged mice.
• To assess the impact of the Si-based agent on sperm parameters, testicular histology, and oxidative stress markers.

Main Methods:

• Aged C57BL/6J mice were fed either a normal diet or a Si-based agent-supplemented diet.
• Sperm analysis, histopathology, and molecular techniques (immunohistochemistry, immunofluorescence, immunoblotting) were employed.
• The study assessed spermatogenic function, oxidative stress, and the Kelch-like ECH-associated protein 1 (KEAP1)/nuclear factor erythroid 2-related factor 2 (NRF2) pathway.

Main Results:

• The Si-based agent significantly enhanced sperm count and motility (curvilinear and straight-line velocity).
• Improved testicular histology and reduced abnormal seminiferous tubules were observed.
• The agent decreased oxidative stress markers and modulated the KEAP1/NRF2 pathway, upregulating antioxidant genes (HMOX-1, NQO-1).

Conclusions:

• Hydrogen delivered via a Si-based agent demonstrates potential as a therapeutic strategy for age-related male infertility.
• Further clinical research is warranted to explore the therapeutic applications of this approach.