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Related Experiment Video

Updated: Sep 13, 2025

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

Published on: December 14, 2015

Optimizing standardized lab-grown skin substitutes evidences a proliferation-differentiation switch based on ascorbic

Angie Katherine Molina-Oviedo¹, Ilaria Sorrentino¹, Irene Clares-Pedrero²

¹Department of Neurosciences and Biomedical Sciences, University Carlos III of Madrid, 28903 Madrid, Spain.

|July 30, 2025

Summary

This summary is machine-generated.

Ascorbic acid (AA) levels regulate skin cell development. Low AA promotes progenitor-like cells and better skin structure, while high AA induces motility and poor stratification in bioengineered skin models.

Keywords:

Biochemistry Biological sciences Cell biology

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Last Updated: Sep 13, 2025

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

Published on: December 14, 2015

Generation of Self-assembled Vascularized Human Skin Equivalents

Generation of Self-assembled Vascularized Human Skin Equivalents

Published on: February 12, 2021

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Published on: May 28, 2021

Area of Science:

Biotechnology
Dermatology
Cell Biology

Background:

Developing bioengineered human skin requires understanding dermal-epidermal interactions.
Metabolites from the dermis can influence epidermal development and cell phenotype.

Purpose of the Study:

To investigate the role of ascorbic acid (AA) as a dermis-donated metabolite in modulating keratinocyte phenotype and epidermal development.
To determine the optimal concentration of AA for achieving desired cellular phenotypes in bioengineered skin constructs.

Main Methods:

Utilized immortalized keratinocytes and organotypic skin cultures.
Administered L-ascorbic acid 2-phosphate (A2P), a stable AA derivative, at varying concentrations.
Assessed cellular phenotypes, including clonogenicity, nuclear/cytoplasmic ratio, progenitor marker expression, and epithelial stratification.

Main Results:

A specific concentration (2 μg/mL A2P) induced a basal-like keratinocyte phenotype with increased progenitor markers and clonogenicity.
Higher A2P concentrations led to intracellular AA accumulation, promoting a motile cell status.
In organotypic cultures, 2 μg/mL A2P improved epithelial layering, while higher doses impaired stratification.

Conclusions:

Ascorbic acid levels critically influence keratinocyte commitment to differentiation versus self-renewal.
Controlled AA levels are essential for achieving functional epidermal architecture in bioengineered skin.
Findings provide insights into optimizing regenerative outcomes for skin tissue engineering.