Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Determining the Plane of Cell Division02:13

Determining the Plane of Cell Division

3.4K
Positioning the cell division plane is a critical step during development and cell differentiation, particularly during mitosis when the plane is essential for determining the size of the two daughter cells. The cell division plane is perpendicular to the plane of chromosome segregation, but different types of organisms have different cell division mechanisms to suit their morphology and function. 
Animal cells
In animal cells, the cleavage furrow forms along the plane of cell division...
3.4K
Subcellular Fractionation01:32

Subcellular Fractionation

7.3K
The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
7.3K
Nuclear Protein Sorting01:34

Nuclear Protein Sorting

4.8K
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
4.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Response to concerns regarding the critical evaluation of 3D-Shaper.

JBMR plus·2026
Same author

Spatially resolved transcriptomic and proteomic profiling reveals cell interaction programs that predict Barrett's esophagus progression.

bioRxiv : the preprint server for biology·2026
Same author

Spatially tunable multiomic sequencing using light-driven combinatorial barcoding of molecules in tissues.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

A toolkit for generating virtual brightfield images of histological and immunohistochemical stains from multiplexed data with AI-based channel selection and image enhancement.

Frontiers in bioinformatics·2026
Same author

Gas-depleted planet formation occurred in the four-planet system around the red dwarf LHS 1903.

Science (New York, N.Y.)·2026
Same author

Critical evaluation of 3D-DXA and 3D-Shaper: methodological limitations and their implications.

JBMR plus·2025
Same journal

Potential role of the <i>Trpv4 c.1491+1G>A</i> mutation in pulmonary fibrosis in a gene-edited mouse model.

Frontiers in genetics·2026
Same journal

Utilization of whole exome sequencing to identify hereditary mutations in Palestinian families with hereditary cancers.

Frontiers in genetics·2026
Same journal

Research of N-acetyl-L-cysteine on CD40-CD40L pathway in pulmonary fibrosis induced by silicon dioxide.

Frontiers in genetics·2026
Same journal

Novel variants in LSS related hypotrichosis simplex 14.

Frontiers in genetics·2026
Same journal

Network-based analysis identifies shared mechanisms between ischemic stroke and myocardial infarction and therapeutic ingredients of Buyang Huanwu Decoction.

Frontiers in genetics·2026
Same journal

GWAS analysis of a depression cohort defined by an EHR-phenotyping algorithm reveals the role of immune regulations in depression risk.

Frontiers in genetics·2026
See all related articles

Related Experiment Video

Updated: Sep 13, 2025

From Voxels to Knowledge: A Practical Guide to the Segmentation of Complex Electron Microscopy 3D-Data
12:08

From Voxels to Knowledge: A Practical Guide to the Segmentation of Complex Electron Microscopy 3D-Data

Published on: August 13, 2014

24.7K

Robust consensus nuclear and cell segmentation.

Melis O Irfan1,2, Eduardo A González-Solares1, Tristan Whitmarsh1

  • 1Institute of Astronomy, University of Cambridge, Cambridge, United Kingdom.

Frontiers in Genetics
|July 30, 2025
PubMed
Summary
This summary is machine-generated.

Biomedical researchers often struggle to select optimal cell segmentation methods. CellSampler software integrates multiple techniques, creating an "uber mask" for improved accuracy in cell segmentation tasks.

Keywords:
bioinformaticscomputer visionimaging mass cytometrymultiplexed imagingsingle-cell segmentation

More Related Videos

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
08:40

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Published on: April 8, 2016

12.9K
Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
11:25

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Published on: February 22, 2016

11.0K

Related Experiment Videos

Last Updated: Sep 13, 2025

From Voxels to Knowledge: A Practical Guide to the Segmentation of Complex Electron Microscopy 3D-Data
12:08

From Voxels to Knowledge: A Practical Guide to the Segmentation of Complex Electron Microscopy 3D-Data

Published on: August 13, 2014

24.7K
Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
08:40

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Published on: April 8, 2016

12.9K
Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
11:25

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Published on: February 22, 2016

11.0K

Area of Science:

  • Biomedical Imaging
  • Computational Biology
  • Image Analysis

Background:

  • Cell segmentation is vital for biomedical research, with many available techniques.
  • Selecting the best method for specific tissues and research goals is time-consuming and resource-intensive for researchers.
  • Current methods require significant expertise and time, diverting focus from primary research objectives.

Purpose of the Study:

  • To develop a software solution that simplifies and optimizes cell segmentation.
  • To create a unified segmentation mask by combining outputs from multiple established methods.
  • To provide researchers with a tool that automates the selection of the best segmentation masks.

Main Methods:

  • Developed CellSampler, a software wrapper for existing cell segmentation algorithms.
  • Implemented a method to combine individual segmentation masks into a single "uber mask".
  • Allowed users to define neighborhood size and statistical measures for selecting the optimal mask components.

Main Results:

  • CellSampler successfully integrates multiple segmentation masks.
  • The "uber mask" approach optimizes segmentation by selecting the best results across local image neighborhoods.
  • User-defined parameters allow for tailored optimization based on specific image characteristics and research needs.

Conclusions:

  • CellSampler streamlines the cell segmentation process for biomedical researchers.
  • The software reduces the time and expertise required for method selection.
  • This tool enhances the efficiency and accuracy of cell segmentation in diverse biomedical imaging applications.