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Preparation of Polyoxometalate-based Photo-responsive Membranes for the Photo-activation of Manganese Oxide Catalysts
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Unidirectional Transmembrane Photoinduced Electron Transfer with Artificial Metallopeptides.

David M Klein1, Xinmeng Li1, Aimee L Boyle1

  • 1Leiden Institute of Chemistry, Leiden University, Leiden 2333 CC, The Netherlands.

Artificial Photosynthesis (Washington, D.C.)
|July 30, 2025
PubMed
Summary

Artificial photosynthesis achieved unidirectional electron transfer across lipid membranes using two metallopeptides. The neutral WALP23-Re2 peptide enabled genuine transfer, while WALP23-Ru2 showed membrane leakage, highlighting WALP23-Re2

Keywords:
artificial photosynthesisleakagelipid bilayersphotoelectron transfertransmembrane

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Area of Science:

  • Artificial photosynthesis
  • Supramolecular chemistry
  • Membrane biophysics

Background:

  • Thylakoid membranes exhibit unidirectional photoinduced electron transfer, a key feature for artificial photosynthesis.
  • Recreating this transmembrane electron transfer artificially is a significant challenge in synthetic biology and materials science.

Purpose of the Study:

  • To design and investigate artificial metallopeptides for driving unidirectional photoinduced electron transfer across lipid membranes.
  • To elucidate the mechanism of transmembrane electron transfer mediated by different metallopeptide structures.

Main Methods:

  • Incorporation of two artificial metallopeptides, WALP23-Re2 and WALP23-Ru2, into dissymmetric liposomes.
  • Utilizing liposomes with an inner electron donor and an outer electron acceptor.
  • Photoirradiation under air to induce and monitor electron transfer.

Main Results:

  • Both WALP23-Re2 and WALP23-Ru2 demonstrated unidirectional electron transfer across the liposome membrane upon light irradiation.
  • The neutral WALP23-Re2 peptide facilitated genuine transmembrane electron transfer.
  • The tetracationic WALP23-Ru2 peptide's activity was attributed to light-induced membrane leakage and subsequent electron transfer, not direct transmembrane transfer.

Conclusions:

  • The neutral metallopeptide WALP23-Re2 shows significant potential for driving transmembrane photoinduced electron transfer in artificial photosynthetic systems.
  • Membrane leakage studies are crucial for validating the mechanisms of artificial transmembrane electron transfer systems.
  • Understanding metal center influence is key to designing efficient artificial photosynthetic devices.