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A convenient S-2-aminoethylation of cysteinyl residues in reduced proteins
Analytical Biochemistry
|September 1, 1985
Abstract:
The cysteinyl residues in proteins had been S-2-aminoethylated with ethylenimine quantitatively. However, ethylenimine is now listed as a carcinogen and is no longer commercially available. A method of converting cysteinyl residues to S-2-aminoethyl derivatives quantitatively using 2-bromoethylamine under mild conditions was developed here.