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Corrigendum to 'A quick-to-implement and optimized CRISPR-Cas9 protocol to obtain insertional and small indel mutants

Mariano A De Silvio1, Camila Sánchez-Retuerta1, M Águila Ruiz-Sola1,2,3

  • 1Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus UAB, Bellaterra 08193 Barcelona, Spain.

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|July 30, 2025
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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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