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Responses to Salt Stress02:02

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Updated: Sep 13, 2025

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
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Soybean Inositol Polyphosphate 5-Phosphatase 8 Confers Salt Tolerance by Reducing Sodium Influx Through Inositol

Qi Jia1,2, Yuan Chen1, Defeng Kong1

  • 1Key Laboratory for Genetics Breeding and Multiple Utilization of Crops, Ministry of Education/College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, China.

Plant, Cell & Environment
|July 30, 2025
PubMed
Summary

Overexpressing the Gs5PTase8 gene enhances plant salt tolerance by reducing sodium accumulation. This inositol polyphosphate 5-phosphatase enzyme plays a key role in maintaining ion homeostasis under salt stress.

Keywords:
calcium (Ca2+) signallinginositol 1,4,5‐trisphosphate (IP3)inositol polyphosphate 5‐phosphatase (5PTase)salt overly sensitive (SOS)salt tolerancesodium (Na+) accumulationsoybean

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Area of Science:

  • Plant Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Inositol metabolism is implicated in plant salt tolerance, but mechanisms remain unclear.
  • Wild soybean (Glycine soja) inositol polyphosphate 5-phosphatase (Gs5PTase8) shows potential for enhancing crop stress resilience.

Purpose of the Study:

  • To investigate the role of Gs5PTase8 in improving salt tolerance in various plant systems.
  • To elucidate the molecular mechanisms underlying Gs5PTase8-mediated salt tolerance, focusing on inositol phosphate signaling.

Main Methods:

  • Ectopic expression of Gs5PTase8 in Arabidopsis thaliana, soybean hairy roots, and composite plants.
  • Measurement of ion content (Na+, K+) under salt stress.
  • Analysis of inositol 1,4,5-trisphosphate (IP3) levels.
  • Complementation experiments with IP3 supplementation in tobacco BY-2 cells.
  • Proteomic analysis using data-independent acquisition.

Main Results:

  • Gs5PTase8 overexpression significantly improved salt tolerance in transgenic plants and tissues.
  • Overexpression reduced sodium (Na+) accumulation and maintained lower Na+/K+ ratios under salt stress.
  • Reduced IP3 levels were observed in Gs5PTase8-overexpressing lines, suggesting 5-phosphatase activity is crucial.
  • External IP3 supplementation reversed the salt tolerance phenotype in Gs5PTase8-overexpressing cells.
  • Proteomic data indicated Gs5PTase8 influences calcium signaling and salt stress response pathways.

Conclusions:

  • Gs5PTase8 enhances plant salt tolerance by modulating inositol phosphate metabolism, specifically by degrading IP3.
  • This degradation likely maintains ion homeostasis and influences key salt stress signaling pathways, contributing to improved plant resilience.
  • Gs5PTase8 represents a promising target for genetic engineering to develop salt-tolerant crops.