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Published on: May 8, 2015
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Solvent-free method for developing latent fingerprints by applying superfine ninhydrin powder.
Yung-Chien Yu1, Hong-Sheng Deng1, Li-Ling Cho2
1Fingerprint Division, Criminal Investigation Bureau, Taipei, Taiwan.
Journal of Forensic Sciences
|July 30, 2025
Summary
A new solvent-free method uses superfine ninhydrin powder (ASNP) for latent fingerprint development on porous surfaces. This approach avoids drawbacks of traditional liquid reagents, offering a safer and more effective alternative for various paper types.
Area of Science:
- Forensic Science
- Analytical Chemistry
- Material Science
Background:
- Ninhydrin is a standard reagent for developing latent fingerprints on porous materials.
- Traditional ninhydrin solutions can damage inks and cause thermal paper to darken.
- Existing methods require pre-prepared solutions, increasing costs and potential hazards.
Purpose of the Study:
- To introduce and evaluate a novel solvent-free method for fingerprint development using superfine ninhydrin powder (ASNP).
- To compare the efficacy of the ASNP method against traditional ninhydrin solutions.
- To assess the ASNP method's performance on diverse paper types, including challenging substrates like thermal and carbonless copy paper.
Main Methods:
- Direct application of finely ground ninhydrin powder to paper surfaces.
- Controlled heating and humidification to promote ninhydrin diffusion into paper fibers.
- Comparative analysis of ASNP and traditional ninhydrin methods under varying temperature and humidity conditions (60°C/40% RH and 50°C/65% RH).
Main Results:
- The ASNP method, particularly at 50°C and 65% RH, significantly improved fingerprint visibility, with prints appearing within 6 hours.
- After 24 hours, the ASNP method's performance was comparable to the traditional ninhydrin solution.
- The ASNP technique demonstrated effectiveness on various paper types, including thermal and carbonless copy paper, with minimal background staining.
Conclusions:
- The solvent-free ASNP method presents a viable and advantageous alternative to traditional ninhydrin solutions for latent fingerprint development.
- This technique eliminates the need for solution preparation, reduces costs, and mitigates solvent-related damage and environmental concerns.
- ASNP is particularly effective for developing fingerprints on sensitive materials like thermal and carbonless copy paper.

