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Updated: Jul 31, 2026

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
Published on: March 13, 2018
Molecular virology profiles of dengue cases in Bandung, Indonesia
Nisa Fauziah1,2,3,4, Yunia Sribudiani1, Lia Faridah1,2
1Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, Indonesia.
Background Objectives:
Bandung City, West Java, Indonesia, is a hyperendemic area for dengue virus (DENV) infections. However, data on virus distribution in this area is currently limited. This study aims to assess the distribution of serotypes and genotypes of DENV isolated from dengue patients in primary healthcare facilities (PHC) in 2018.
Methods:
A cross-sectional study was conducted in three PHCs in Bandung City from May to October 2018. Suspected dengue patients with a fever lasting more than five days were enrolled. Demographic and clinical data were collected upon enrollment. Dengue NS1 rapid diagnostic test (RDT) was followed by RT-PCR and nested PCR performed on patients' serum samples. A phylogenetic tree was constructed based on E-gene sequence data.
Results:
A total of 236 suspected dengue patients were enrolled in this study, with 51 confirmed dengue infections based on the NS1 rapid test. Among these 51 cases, 23 (45%) were positive by RT-PCR (2 DENV-1, 6 DENV-2, 10 DENV-3, 3 DENV-4, and 2 with mixed DENV serotypes). The predominance DENV-3 in 2018 was consistent with the reports from 2008, indicating the same predominant serotype circulated in the area. Phylogenetic analysis revealed that DENV-1 genotype 1 and DENV-3 genotype 1 were circulating in Bandung.
Interpretation Conclusion:
All four DENV serotypes were identified in samples collected from PHC Neglasari, Padasuka, and Sukajadi, confirming that Bandung is a hyperendemic area. Strengthening vector control measures is crucial to prevent further transmission of dengue infection.
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