Related Experiment Video
Updated: Sep 13, 2025

Nucleocapsid Annealing-Mediated Electrophoresis NAME Assay Allows the Rapid Identification of HIV-1 Nucleocapsid Inhibitors
Published on: January 19, 2015
Binding of SARS-CoV-1/2 NSP1 to DNA Polymerase α-Primase Inhibits DNA Replication through Reduction of Interaction
Hung Van Nguyen1, Nguyen Le Ngoc Lan2, Mai Suan Li1
1Institute of Physics, Polish Academy of Sciences, Al. Lotnikow 32/46, 02-668 Warsaw, Poland.
Abstract:
A comprehensive understanding of the atomic level mechanism governing the binding nonstructural protein 1 of SARS-CoV-1 (SARS-CoV-1 NSP1) and SARS-CoV-2 (SARS-CoV-2 NSP1) to Pol α-primase is important to advance the development of small molecule inhibitors for the treatment COVID-19. In this study, we use both all-atom steered molecular dynamics (all-atom SMD) and coarse-grained umbrella sampling (coarse-grained US) simulations to assess the binding affinity of SARS-CoV-1 NSP1 and SARS-CoV-2 NSP1 to Pol α-primase. Our all-atom SMD and coarse-grained US simulations consistently indicate that SARS-CoV-2 NSP1 exhibits stronger affinity for Pol α-primase compared to SARS-CoV-1 NSP1, implying that SARS-CoV-2 poses a greater risk than SARS-CoV-1 in impeding DNA replication for DNA synthesis. Through an energetic decomposition analysis of the interaction energies within these complexes, we identify electrostatic interactions as the primary contributors to the observed difference in binding affinity. We found that hydrogen bonds between Asp33 and Arg616 in SARS1 NSP1-Pol α-primase, and Asp33 with Arg616 and Lys655 in SARS2 NSP1-Pol α-primase, are critical for the interaction of both SARS-CoV-1 NSP1 and SARS-CoV-2 NSP1 with Pol α-primase. Asp33 in SARS-CoV-2 NSP1 shows increased solubility and stability compared to SARS-CoV-1 NSP1, enhancing its association with Pol α-primase. This finding lays the groundwork for innovative strategies aimed at inhibiting the interaction between these entities, offering promising avenues for therapeutic intervention against COVID-19. We also estimated the binding free energy of DNA to Pol α-primase, SARS1 NSP1-Pol α-primase, and SARS2 NSP1-Pol α-primase using the MM-PBSA method. The results show the order: Pol α-primase-DNA < SARS1 NSP1-Pol α-primase-DNA < SARS2 NSP1-Pol α-primase-DNA, indicating that both SARS-CoV-1 NSP1 and SARS-CoV-2 NSP1 reduce DNA binding to Pol α-primase, suggesting impaired DNA synthesis.
More Related Videos
Related Concept Videos
Restarting Stalled Replication Forks
Single-Strand DNA Binding Proteins
S-Cdk Initiates DNA Replication
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of...
DNA Damage can Stall the Cell Cycle
The DNA Replication Fork
Negative Regulator Molecules

