Schisandrol A-loaded mesenchymal stem cell exosomes modulate FTO/miR-21/WNT5A axis to promote skin healing

Schisandrol A (SA), the primary active component of Schisandra chinensis, exhibits antioxidant properties, safeguarding cells from oxidative damage. However, its effect on wound healing remains elusive, and its low water solubility restricts its utilization efficiency.

This study aimed to explore the impact of SA on HaCaT cell proliferation and migration through in vitro experiments, thereby providing deeper insights into its role in skin wound healing.

In vitro cell experiments and in vivo mouse wound model experiments were employed to investigate the effects of SA on cells and wound healing.

1. In vitro Experiments: HaCaT cells were used to observe the influence of SA on their proliferation and migration. 2. RNA Sequencing: RNA sequencing was utilized to determine the molecular mechanism underlying the healing effect of SA, specifically the regulation of miR-21/WNT5A expression by FTO. 3. Animal Experiments: In a mouse wound model, PF-127 hydrogel was used as a dressing for the delivery of exosomes and SA. The wounds of the group treated with SA alone were compared with those of the group treated with SA-carrying exosomes.

1. SA enhanced HaCaT cell migration and proliferation. 2. RNA sequencing indicated that the healing effect of SA was mediated by the regulation of miR-21/WNT5A expression via FTO. 3. In the mouse wound model, the group treated with SA-carrying exosomes demonstrated enhanced wound-healing speed and efficacy than in the group treated with SA alone. These results were further confirmed by histopathological and biochemical analyses.

Exo-SA modulates the FTO/miR-21/WNT5A axis to promote skin healing.