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Updated: Sep 13, 2025
06:17
A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue
Published on: August 25, 2018
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Supersulfides regulate cell migration in human skin keratinocytes
Kento Kunihiro1, Katsura Sano1
1ALBION Co., Ltd.
Cell Structure and Function
|July 30, 2025
Summary
Supersulfides are present in skin and promote keratinocyte migration, crucial for wound healing. This study reveals their novel role in re-epithelialization and potential as a therapeutic target for skin injuries.
Area of Science:
- Biochemistry and Molecular Biology
- Dermatology
- Wound Healing Research
Background:
- The skin's outermost layer is vulnerable to damage, with keratinocyte migration being vital for wound repair.
- Supersulfides are known for energy production but their role in skin physiology and wound healing remains largely unexplored.
Purpose of the Study:
- To investigate the presence and function of supersulfides in skin.
- To determine the impact of supersulfides on keratinocyte migration during wound healing.
Main Methods:
- Utilized sulfane sulfur probe 4 (SSP4) to detect supersulfides in skin tissues and keratinocytes.
- Examined the expression of cysteinyl-tRNA synthetase 2 (CARS2), the key enzyme in supersulfide biosynthesis.
- Performed CARS2 knockdown and Na2S4 treatment experiments in keratinocytes.
Main Results:
- Supersulfides and CARS2 were detected in skin and keratinocytes, with increased levels during wound healing.
- CARS2 knockdown inhibited keratinocyte migration and downregulated chemokine gene expression.
- Supersulfides were found to regulate E-cadherin and MMP-9 via ERK and Akt signaling pathways, with Na2S4 restoring migration in CARS2-knockdown cells.
Conclusions:
- Supersulfides play a significant role in regulating keratinocyte migration and skin wound re-epithelialization.
- Supersulfides represent a novel therapeutic target for enhancing skin wound healing.
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