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Updated: Sep 13, 2025

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
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Third-generation novel technologies for gene editing.

Pushpendra K Gupta1, Sourabh Kumar1

  • 1Department of Genetics and Plant Breeding, Chaudhary Charan Singh University, Meerut, India.

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|July 30, 2025
PubMed
Summary
This summary is machine-generated.

Third-generation gene editing technologies like seekRNA and bridgeRNA overcome limitations of CRISPR-Cas systems. These advanced tools address issues such as protospacer adjacent motif requirements and double-strand breaks for improved gene editing applications.

Keywords:
CASTCRISPRCas3OMEGAPASSIGEPASTEgene editingseekRNA/bridgeRNA

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Genomics

Background:

  • CRISPR-Cas systems are widely used for gene editing in crops and therapeutics.
  • Existing CRISPR-Cas methods have limitations including protospacer adjacent motif dependence and double-strand break generation.

Purpose of the Study:

  • To review third-generation gene editing technologies.
  • To highlight advancements beyond CRISPR-Cas, base editors, and prime editors.

Main Methods:

  • Review of scientific literature on gene editing technologies.
  • Focus on seekRNA and bridgeRNA systems.

Main Results:

  • Second-generation editors (base editors and prime editors) partially addressed CRISPR-Cas limitations.
  • Third-generation technologies offer solutions to remaining challenges in gene editing.

Conclusions:

  • seekRNA and bridgeRNA represent significant advancements in gene editing.
  • These novel technologies hold promise for overcoming current gene editing hurdles.