Related Experiment Video
Updated: Sep 13, 2025

Determining 3'-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells
Published on: January 30, 2019
1H, 13C and 15N chemical shift assignment for stem-loop 5a from the 5'UTR of HCoV-229E
Nina M Krause1,2, Anna Wacker1,2, Christian Richter1,2
1Institute for Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe University, Max-von-Laue-Straße7, 60438, Frankfurt/M, Germany.
Abstract:
Due to the emergence of the SARS-CoV-2 virus, research on coronaviruses has been massively accelerated. In addition to SARS-CoV-2, there are other human coronaviruses, including HCoV-229E. In all coronaviruses, secondary structure predictions indicate the presence of conserved structural elements in the 5'-untranslated region (5'-UTR). These conserved elements play crucial roles in RNA translation and replication. Stem-loop 5 (SL5), consisting of three substructures (5a, 5b, 5c), is highly conserved and harbours the start codon for translation. SL5 has repetitive structural motifs (RSMs), 5'-UUYYGU-3', which are conserved in many alpha- and betacoronaviruses. In the following, we present the 1H, 13C and 15N NMR resonance assignment of the SL5a RNA element from HCoV-229E and variations in the RSMs to show the effect of loop mutations on the structure of the hexaloop, revealing the different impact of each loop nucleotide on RNA dynamics.
Related Concept Videos
Cis-regulatory Sequences
Leaky Scanning
Position-effect Variegation
Point and Frameshift Mutations
Mismatch Repair
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
¹H NMR Chemical Shift Equivalence: Homotopic and Heterotopic Protons

