Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Nitrogen Metabolism01:20

Overview of Nitrogen Metabolism

8.5K
Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of  ammonia, ammonium ions, nitrate, nitrite, or  nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
8.5K
The Nitrogen Cycle01:49

The Nitrogen Cycle

54.3K
Nitrogen atoms, present in all proteins and DNA, are recycled between abiotic and biotic components of the ecosystem. However, the primary form of nitrogen on Earth is nitrogen gas, which cannot be used by most animals and plants. Thus, nitrogen gas must first be converted into a usable form by nitrogen-fixing bacteria before it can be cycled through other living organisms. The use of nitrogen-containing fertilizers and animal waste products in human agriculture has greatly influenced the...
54.3K
Inorganic Nitrogen Assimilation01:22

Inorganic Nitrogen Assimilation

108
Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
108
Metabolism of Chemolithotrophs01:15

Metabolism of Chemolithotrophs

176
Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation.
176
Comparative Excretory Systems02:24

Comparative Excretory Systems

23.7K
Animals have evolved different strategies for excretion, the removal of waste from the body. Most waste must be dissolved in water to be excreted, so an animal’s excretory strategy directly affects its water balance.
23.7K
Free Energy Changes for Nonstandard States03:25

Free Energy Changes for Nonstandard States

11.6K
The free energy change for a process taking place with reactants and products present under nonstandard conditions (pressures other than 1 bar; concentrations other than 1 M) is related to the standard free energy change according to this equation:
 
where R is the gas constant (8.314 J/K·mol), T is the absolute temperature in kelvin, and Q is the reaction quotient. This equation may be used to predict the spontaneity of a process under any given set of conditions.
Reaction Quotient...
11.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Direct air capture technologies: innovations, integration, and pathways to scale.

Chemical Society reviews·2026
Same author

A Comparative Review of Biological, Electrochemical, and Membrane-Based Methods for Direct Ocean Carbon Capture.

Materials (Basel, Switzerland)·2026
Same author

Catalytic hybrid solvent regeneration in membrane vacuum processes for direct air capture.

Nature communications·2026
Same author

Printed Liquid Metal-Solid Metal Hybrid Electrodes for Stabilizing Liquid Platinum-Gallium Droplets During Electrocatalysis.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Engineering Temperature-Switchable Conducting Metal-Phenolic Network Films.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Liquid Metal Printed Zinc Tin Composite Oxide Nanosheets: A Platform for Multifunctional Sensing at Room Temperature.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025

Related Experiment Video

Updated: Sep 13, 2025

Ammonia Synthesis at Low Pressure
08:14

Ammonia Synthesis at Low Pressure

Published on: August 23, 2017

26.7K

Carbon-Negative Ammonia Production from the Air.

Dingqi Wang1, Xue Yan2, Jining Guo1

  • 1Department of Chemical Engineering, University of Melbourne, Parkville, Victoria, 3010, Australia.

Angewandte Chemie (International Ed. in English)
|July 31, 2025
PubMed
Summary

This study introduces a novel, carbon-negative ammonia synthesis method using air. The process captures atmospheric carbon dioxide (CO2) and produces ammonia sustainably, offering a greener alternative to traditional methods.

Keywords:
Carbon storageCarbon‐negative ammonia synthesisEnergy saving direct air captureGreen ammonia productionNitrogen fixation

More Related Videos

Calibrated Passive Sampling - Multi-plot Field Measurements of NH3 Emissions with a Combination of Dynamic Tube Method and Passive Samplers
10:29

Calibrated Passive Sampling - Multi-plot Field Measurements of NH3 Emissions with a Combination of Dynamic Tube Method and Passive Samplers

Published on: March 21, 2016

12.4K
Electrochemically and Bioelectrochemically Induced Ammonium Recovery
09:50

Electrochemically and Bioelectrochemically Induced Ammonium Recovery

Published on: January 22, 2015

12.8K

Related Experiment Videos

Last Updated: Sep 13, 2025

Ammonia Synthesis at Low Pressure
08:14

Ammonia Synthesis at Low Pressure

Published on: August 23, 2017

26.7K
Calibrated Passive Sampling - Multi-plot Field Measurements of NH3 Emissions with a Combination of Dynamic Tube Method and Passive Samplers
10:29

Calibrated Passive Sampling - Multi-plot Field Measurements of NH3 Emissions with a Combination of Dynamic Tube Method and Passive Samplers

Published on: March 21, 2016

12.4K
Electrochemically and Bioelectrochemically Induced Ammonium Recovery
09:50

Electrochemically and Bioelectrochemically Induced Ammonium Recovery

Published on: January 22, 2015

12.8K

Area of Science:

  • Chemical Engineering
  • Materials Science
  • Environmental Science

Background:

  • Ammonia is crucial for fertilizers and hydrogen storage.
  • Conventional ammonia synthesis (Haber-Bosch) is energy-intensive and relies on fossil fuels, contributing to greenhouse gas emissions.
  • There is a critical need for sustainable and carbon-negative ammonia production methods.

Purpose of the Study:

  • To develop a novel process for synthesizing ammonia directly from atmospheric nitrogen.
  • To integrate direct air capture (DAC) of carbon dioxide (CO2) into the ammonia synthesis pathway.
  • To establish a sustainable and carbon-negative technology for ammonia production.

Main Methods:

  • Utilized lithium as a mediator for nitrogen fixation via nitridation at ambient pressure and below 80°C.
  • Generated ammonia by exposing the nitride intermediate to moisture.
  • Integrated direct air capture of CO2 using the co-produced lithium hydroxide intermediate.
  • Recycled lithium and released pure CO2 via electrolysis of lithium carbonate.

Main Results:

  • Successfully synthesized ammonia from atmospheric nitrogen using a low-temperature, ambient-pressure lithium-mediated process.
  • Achieved direct air capture of CO2 with a high capacity of 18.4 mmol CO2/g.
  • Demonstrated the recycling of lithium and release of pure CO2, confirming a closed-loop system.
  • Established a proof-of-concept for a carbon-negative ammonia synthesis technology.

Conclusions:

  • The developed process offers a sustainable pathway for ammonia production from air.
  • This technology effectively integrates ammonia synthesis with direct air capture, leading to carbon negativity.
  • This work presents a significant advancement towards sustainable chemical production and negative-emission technologies.