Aerobic bacterial flora from dead-in-shell chicken embryos from Nigeria

Dead-in-shell chicken embryos from two commercial hatcheries in Anambra State of Nigeria were investigated for isolation of aerobic bacteria. For this purpose, 79 pooled samples containing 632 dead-in-shell chicken embryos were cultured. From these samples, 23 isolates of Escherichia coli and 25 isolates of Staphylococcus aureus were recorded. Other bacterial species isolated included Micrococcus sp. (fifteen isolates), Klebsiella sp. (thirteen isolates), Pseudomonas sp. (nine isolates), and Proteus sp. (seven isolates). Salmonella, Streptococcus, and Mycoplasma spp. could not be isolated. A high incidence of pathogenic strains of bacteria from dead-in-shell chicken embryos was observed. This suggests that the isolates may have contributed to the embryonic mortality and reduced hatchability recorded in the farms investigated.