Temperature-Sensitive Lipids Reveal Intraspecific Diversity in Bacteria Isolated from an Ancient Antarctic Microbial...

Ancient Antarctic microbial mats harbour viable bacteria that have been exposed to extreme cold and arid conditions for hundreds of years. To delve into the molecular mechanisms underlaying their survival, we isolated 12 bacterial strains from a 1,000-year-old desiccated microbial mat from the McMurdo Ice Shelf and studied their lipid composition as a function of temperature. Six of the bacterial strains were classified as Paenisporosarcina macmurdoensis, and the other six as Arthrobacter sp., according to 16S rRNA gene analyses. Two strains of each taxon were incubated at their minimum and maximum growth temperatures, and the changes in their lipid profiles were analyzed. The proportion of major fatty acids (saturated, unsaturated, and iso- and anteiso-) remained relatively constant across temperature in the four strains, but the composition of hydrocarbons and alcohols differed between taxa (e.g., presence of unsaturated alkenes in Arthrobacter sp., or unidentified isoprenoid alcohols in P. macmurdoensis). This highlights the diagnostic value of non-fatty acid lipids and revealed a taxon-dependent lipid composition. Despite the taxon-associated lipid profile, incubation temperature also influenced lipid composition in both taxa, with higher temperature correlating with greater lipid richness. Interestingly, the two P. macmurdoensis strains showed distinct lipid profiles at 20°C, suggesting that intraspecific lipid diversity reflects within-species physiological variability with potential relevance for adaptation to temperature fluctuations in the mat. Therefore, assessing the influence of temperature on bacterial lipids is crucial for understanding their adaptation and survival in extreme environments, as well as for expanding species lipid inventories for biological interpretations of ancient samples.