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Related Experiment Video

Updated: Sep 13, 2025

Isolation and Decellularization of a Whole Porcine Pancreas
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Protocol for decellularizing and delipidizing human pancreatic tissue into native hydrogel.

Connie S Chamberlain1, Daniel M Tremmel1, Ayesha Palwasha Khan1

  • 1Division of Transplantation, Department of Surgery, University of Wisconsin-Madison, Madison, WI 53705, USA.

STAR Protocols
|July 31, 2025
PubMed
Summary

This study details a protocol to create human pancreatic extracellular matrix (ECM) hydrogels. The method involves decellularization, delipidization, and digestion for scaffold applications.

Keywords:
Biotechnology and bioengineeringOrganoidsTissue Engineering

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Last Updated: Sep 13, 2025

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Biochemistry

Background:

  • The extracellular matrix (ECM) is crucial for tissue structure and function.
  • Developing functional biomaterials from native tissues is essential for regenerative medicine.
  • Pancreatic ECM holds potential for therapeutic applications due to its unique composition.

Purpose of the Study:

  • To establish a reproducible protocol for processing human pancreatic tissue into a decellularized and delipidized extracellular matrix (ECM).
  • To prepare pancreatic ECM-derived hydrogels and scaffolds for potential biomedical applications.
  • To provide a detailed methodology for researchers in tissue engineering and regenerative medicine.

Main Methods:

  • Human pancreatic tissue undergoes decellularization using sodium deoxycholate.
  • Delipidization is achieved through sequential washes with ethanol, acetone, and hexane.
  • Nucleic acids are removed using benzonase, followed by lyophilization for preservation and pepsin-HCl digestion to form a hydrogel.

Main Results:

  • A comprehensive protocol for generating human pancreatic ECM is presented.
  • The method yields a decellularized and delipidized ECM suitable for further processing.
  • Successful digestion of pancreatic ECM into a hydrogel format is demonstrated.

Conclusions:

  • This protocol provides a reliable method for creating pancreatic ECM-based hydrogels.
  • The developed ECM biomaterial can be utilized for advanced scaffold applications in tissue engineering.
  • Further research can explore the therapeutic potential of these pancreatic ECM hydrogels.