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Microvesicles derived from activated T cells promote human mast cell migration via the S1P1 receptor

Noam Yishay1,2, Yoseph A Mekori1,2, Irit Shefler1,2

  • 1The Herbert Mast Cell Disorders Center, Laboratory of Allergy and Clinical Immunology, Meir Medical Center, Tchernichovsky 59 St, Kfar Saba 4428164, Israel.

Journal of Leukocyte Biology
|July 31, 2025
PubMed

Insights

Activated T-cell microvesicles guide mast cells to inflammation sites. These microvesicles promote mast cell migration via specific signaling pathways, crucial for T-cell-mediated inflammatory responses.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Mast cell activation is observed during T-cell-mediated inflammation.
  • Microvesicles from activated T cells induce mast cell degranulation and cytokine release via the MAPK pathway.

Purpose of the Study:

  • To investigate if microvesicles from activated T cells promote mast cell migration.
  • To elucidate the molecular mechanisms underlying this migration.

Main Methods:

  • Isolation of microvesicles from activated or resting T-cell supernatants.
  • Measurement of mast cell migration using transwell assays.
  • Analysis of molecular mechanisms using specific inhibitors and pathway analysis.

Main Results:

  • Microvesicles from activated T cells significantly enhanced human mast cell chemotaxis.
  • Migration was dependent on ERK and p38 phosphorylation, but not PI3K.
  • The S1P1 receptor and sphingosine kinase 1 mediated the migration.

Conclusions:

  • Microvesicles from activated T cells act as chemoattractants for mast cells.
  • This process is critical for directing mast cells to inflammatory sites.
  • These findings highlight the role of T-cell microvesicles in T-cell-mediated inflammation.
Keywords:
T cellsmast cellsmicrovesiclesmigration

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