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Selective Interfacial Assembled Asymmetric Porous Nanomotors as Maneuverable Labels for Boosting Capture and
Xudong Jing1,2, Xinyue Zhang1,2, Jing Huang3
1The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, P. R. China.
Analytical Chemistry
|July 31, 2025
Summary
Smart nanomotors boost immunosensor sensitivity for early cancer detection. These self-propelling labels significantly improve antigen capture and detection limits, aiding in diagnostics.
Area of Science:
- Nanotechnology and Materials Science
- Biosensing and Immunoassay Development
- Biomedical Engineering and Diagnostics
Background:
- Integrating autonomous motion and multifunctional labels into immunosensors enhances antigen capture and detection sensitivity.
- Current immunosensors face limitations in early-stage detection due to low analyte concentrations and complex sample matrices.
Purpose of the Study:
- To design and fabricate smart asymmetric porous nanomotors (FPSP) for enhanced immunosensor performance.
- To evaluate the efficacy of FPSP nanomotor labels in improving antigen capture efficiency and detection sensitivity for breast cancer biomarkers.
Main Methods:
- Fabrication of FPSP nanomotors using a selective interfacial assembly strategy, endowing them with magnetic, photothermal, porous, and catalytic properties.
- Utilizing near-infrared irradiation to induce thermophoretic motion of FPSP nanomotors via a localized thermal gradient.
- Integration of FPSP nanomotors as labels in a dual-antibody sandwich immunosensor for carbohydrate antigen 153 (CA153) detection.
Main Results:
- FPSP nanomotors demonstrated enhanced collision probability with CA153, increasing capture efficiency by 95.8% compared to static labels.
- The FPSP-based immunosensor achieved an ultrasensitive detection limit of 0.0021 U mL⁻¹, approximately 316.5-fold improvement over static labels.
- FPSP exhibited excellent colorimetric signal brightness, rapid motion, high peroxidase-mimicking activity, and magnetic separation capabilities.
Conclusions:
- Smart asymmetric porous nanomotors (FPSP) effectively overcome sensitivity limitations in immunosensors, particularly for early cancer detection.
- The FPSP-integrated immunosensor offers a significant advancement in biosensing, enabling ultrasensitive quantification of biomarkers like CA153.
- This technology holds promise for applications in cancer diagnostics, environmental monitoring, and other fields requiring high-sensitivity detection.
