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Related Concept Videos

CRISPR01:59

CRISPR

52.9K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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What is Genetic Engineering?00:49

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Overview
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Related Experiment Video

Updated: Sep 13, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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Genome Editing in Mammalian Cell Lines using CRISPR-Cas

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Live genome imaging by CRISPR engineering: progress and problems.

Eui-Jin Park1, Hajin Kim2

  • 1Department of Biomedical Engineering, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea.

Experimental & Molecular Medicine
|July 31, 2025
PubMed
Summary
This summary is machine-generated.

CRISPR-Cas genome imaging now visualizes nonrepetitive DNA in living cells. Advancements improve signal but challenges like cellular toxicity and genomic instability persist, requiring further solutions.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • CRISPR-Cas genome imaging enables visualization of DNA in living cells.
  • Imaging repetitive genomic loci (e.g., centromeres) is established.
  • Imaging nonrepetitive genomic loci remains a significant challenge.

Purpose of the Study:

  • To review recent advancements in CRISPR-Cas-based genome imaging techniques.
  • To discuss the application of these techniques for imaging nonrepetitive genomic loci.
  • To highlight current challenges and potential solutions.

Main Methods:

  • Advancements in CRISPR RNA and Cas protein design.
  • Development of novel fluorophores for enhanced signal detection.
  • Integration of CRISPR-Cas with other molecular machinery to amplify signals and reduce background.

Main Results:

  • Techniques now enable tracking genomic loci with minimal CRISPR-Cas complexes (down to single complexes).
  • Successful application of advanced CRISPR-Cas systems for imaging nonrepetitive genomic loci.
  • Identification of cellular toxicity and genomic instability as key challenges.

Conclusions:

  • CRISPR-Cas genome imaging has revolutionized DNA visualization, extending to nonrepetitive loci.
  • Expression of CRISPR-Cas can induce cellular toxicity and interfere with DNA metabolism.
  • Overcoming adverse effects is crucial for the safe and effective application of CRISPR-Cas genome labeling.