Related Experiment Video
Updated: Sep 13, 2025
07:56
Genome Editing in Mammalian Cell Lines using CRISPR-Cas
Published on: April 11, 2019
22.1K
Applications of multiplexed CRISPR-Cas for genome engineering
Himchan Cheng1, Euihwan Jeong2, Seung Woo Cho3,4
1Department of Biomedical Engineering, College of Information and Biotechnology, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea.
Experimental & Molecular Medicine
|July 31, 2025
Summary
The CRISPR-Cas system enables precise, efficient multiplexed genome editing for various applications. This technology facilitates gene knockout, structural variation induction, epigenetic modification, and targeted cell killing in recent studies.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- The CRISPR-Cas system is a revolutionary genome editing tool with broad applications in research and medicine.
- Its precision and efficiency stem from modulation by a short guide RNA, making it ideal for complex editing tasks.
Purpose of the Study:
- To review the applications of CRISPR-based editing technologies for multiple targets in recent scientific studies.
- To highlight the versatility of CRISPR-Cas for advanced genome manipulation and therapeutic strategies.
Main Methods:
- Simultaneous targeting of multiple genes using the CRISPR-Cas system.
- Utilizing engineered CRISPR-Cas proteins for gene expression modulation (epigenetic editing).
- Inducing DNA damage for targeted cell killing.
Main Results:
- CRISPR-Cas enables efficient gene knockout with large deletions and induction of complex structural variations (inversions, translocations, duplications).
- Multiplexed epigenetic editing is achievable through engineered CRISPR-Cas proteins.
- Simultaneous DNA damage accumulation allows for targeted killing of specific cell types.
Conclusions:
- CRISPR-Cas multiplexed editing offers powerful capabilities for basic research, drug discovery, and therapeutic interventions.
- The technology supports complex genomic modifications and precise cellular manipulation, advancing various biological and medical fields.
Related Concept Videos
01:28CRISPR/Cas9 Genome Editing
252
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
252
01:59CRISPR
52.9K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.9K
02:53CRISPR and crRNAs
17.4K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.4K
02:31Homologous Recombination
52.2K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
52.2K