Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Mapping the LVAD landscape: insights from the ITAMACS registry.

European journal of heart failure·2026

Same author

A machine learning model for early risk stratification of 28-day mortality after myocardial infarction.

European heart journal. Digital health·2026

Same author

Extra-Mitral Abnormalities in Non-Syndromic Mitral Valve Prolapse assessed by Cardiovascular Magnetic Resonance.

The Canadian journal of cardiology·2026

Same author

Dipeptidyl peptidase 3 sets the threshold for immune activation and survival during experimental bacterial infection.

Nature communications·2026

Same author

One-year outcomes of bioresorbable magnesium scaffold implantation in complex coronary lesions.

Frontiers in cardiovascular medicine·2026

Same author

Outcomes after tricuspid transcatheter edge-to-edge repair: a systematic review and meta-analysis.

Annals of cardiothoracic surgery·2026

Search research articles

Related Experiment Video

Updated: Sep 13, 2025

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

Single-cell RNA sequencing of platelets: challenges and potential.

Giacomo Viggiani^1,2, Kilian Kirmes^1,2, Jiaying Han^1,2

¹Department of Internal Medicine I, University Hospital Rechts Der Isar, Technical University of Munich, Munich, Germany.

Journal of Thrombosis and Thrombolysis

|July 31, 2025

Summary

Single-cell RNA sequencing (scRNA-seq) of platelets is now feasible. This pilot study demonstrates a new method for analyzing platelet RNA, offering insights into platelet biology and disease.

Keywords:

10X genomics Platelet rich plasma Platelets Single-cell RNA sequencing Thrombocyte biology

More Related Videos

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Published on: May 22, 2017

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Related Experiment Videos

Last Updated: Sep 13, 2025

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Published on: May 22, 2017

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Area of Science:

Hematology
Molecular Biology
Genomics

Background:

Platelets are vital anuclear cells involved in hemostasis, immunity, and vascular diseases.
Platelets contain RNA inherited from megakaryocytes and can synthesize proteins, but studying their transcriptome is challenging due to limited RNA content and high reactivity.
Previous transcriptomic studies of platelets faced limitations, hindering deep biological insights.

Purpose of the Study:

To establish the feasibility of performing single-cell RNA sequencing (scRNA-seq) on platelets derived from whole blood.
To explore platelet heterogeneity and gene expression profiles at the single-cell level.

Main Methods:

Peripheral whole blood was collected and processed to obtain platelet-rich plasma (PRP).
Single-cell RNA sequencing (scRNA-seq) was performed on PRP using the 10X Genomics platform.
Bioinformatic analyses included data normalization, Uniform Manifold Approximation and Projection (UMAP) clustering, and differential gene expression analysis.

Main Results:

The scRNA-seq successfully identified three distinct platelet clusters.
One cluster was notably enriched for platelet-specific lineage markers, including PPBP and PF4.
Mitochondrial RNA constituted approximately 14% of the total RNA counts.

Conclusions:

This pilot study demonstrates the feasibility of scRNA-seq for platelets isolated from whole blood, overcoming previous technical challenges.
This advancement opens new avenues for understanding platelet biology, immune responses, and vascular diseases at a single-cell resolution.
The findings encourage further research focusing on platelet transcriptomics for clinical applications.