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Size-Exclusion Chromatography

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
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Related Experiment Video

Updated: Sep 13, 2025

$Quantitative Locomotion Study of Freely Swimming Micro-organisms Using Laser Diffraction$

Quantitative Locomotion Study of Freely Swimming Micro-organisms Using Laser Diffraction

Published on: October 25, 2012

Microswimmer separation in complex confining geometries.

Ian P Madden¹, Erik Luijten²

¹Northwestern University, Department of Materials Science and Engineering, Evanston, Illinois 60208, USA.

Physical Review. E

|August 1, 2025

Summary

This summary is machine-generated.

Researchers developed numerical simulations to separate microswimmers based on their propulsion. This study offers insights into designing filters for active colloid separation using hydrodynamic interactions in confined spaces.

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Preparation and 3D Tracking of Catalytic Swimming Devices

Preparation and 3D Tracking of Catalytic Swimming Devices

Published on: July 1, 2016

Area of Science:

* Physics
* Materials Science
* Chemical Engineering

Background:

* Microswimmers, both biological and synthetic, display complex collective behaviors like aggregation and phase separation.
* Separation of confined microswimmer mixtures with different propulsion mechanisms is not well understood.
* Hydrodynamic forces are crucial for controlling separation but are challenging to resolve in complex geometries.

Purpose of the Study:

* To numerically model the separation of microswimmer mixtures with different propulsion styles.
* To investigate the role of hydrodynamic interactions and confining geometries in species separation.
* To design activity-based separation filters for active colloids.

Main Methods:

* Employed massively parallel colloidal hydrodynamic simulations.
* Modeled mixtures of
pushers
and
pullers
microswimmers.
* Systematically varied confining geometries to create separation filters.

Main Results:

* Demonstrated that hydrodynamic interactions with surfaces drive the separation of pusher and puller microswimmers.
* Identified specific confining geometries that effectively filter microswimmer species.
* Quantified the relationship between geometry and separation efficiency.

Conclusions:

* Hydrodynamic interactions in confined environments are key to microswimmer separation.
* The study provides a framework for designing novel microswimmer separation devices.
* Findings advance the field of active colloid manipulation and separation technologies.