Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular Pathogenesis of and Regenerative Strategies for Osteonecrosis of the Femoral Head.

Tissue engineering. Part B, Reviews·2026
Same author

Immunohistochemical expression of SPARC and MMP-9 in odontogenic keratocysts and orthokeratinized odontogenic cysts.

Archives of oral biology·2026
Same author

Expert consensus on the diagnosis and management of tooth developmental anomalies.

International journal of oral science·2026
Same author

Intervertebral disc puncture predisposes the disc to Cutibacterium acnes infection via hematogenous and contiguous routes: a rat model study.

Journal of orthopaedic surgery and research·2026
Same author

Spermidine promotes osteogenic differentiation of BMSCs via the m<sup>6</sup>A methylation-autophagy axis under inflammatory stress.

Free radical biology & medicine·2026
Same author

Unilateral biportal endoscopic with modified arcocristectomy for pinching cervical spondylotic myelopathy: surgical technique and early experiences.

Frontiers in surgery·2025

Related Experiment Video

Updated: Sep 13, 2025

Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells
03:45

Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells

Published on: May 5, 2023

2.5K

Dental pulp stem cells conditioned medium-functionalized microspheres for endodontic regeneration.

Jing Gao1,2, Yuhan Wang1,2, Tongxing Zhang3

  • 1Department of International VIP Dental Clinic, Tianjin Stomatological Hospital, School of Medicine, Nankai University, Tianjin, China.

Frontiers in Cell and Developmental Biology
|August 1, 2025
PubMed
Summary

This study developed nucleus pulposus microspheres (NPM) to mimic the dental pulp microenvironment, enhancing dental pulp stem cell (DPSC) regeneration. The novel approach successfully promoted pulp-like tissue regeneration in vivo.

Keywords:
conditioned mediumdental pulp stem cellsendodontic regenerationmicrospheresodontogenic differentiation

More Related Videos

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
14:52

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

26.7K
Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth
02:33

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth

Published on: May 17, 2024

1.8K

Related Experiment Videos

Last Updated: Sep 13, 2025

Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells
03:45

Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells

Published on: May 5, 2023

2.5K
Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
14:52

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

26.7K
Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth
02:33

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth

Published on: May 17, 2024

1.8K

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Endodontics

Background:

  • Dental pulp disease is common, with limited functional regeneration of the pulp-dentin complex.
  • Stem cell-based endodontic regenerative therapy is promising but faces challenges in recreating the native pulp microenvironment.

Purpose of the Study:

  • To develop a novel strategy using nucleus pulposus microspheres (NPM) loaded with conditioned medium (CM) to mimic the dental pulp regeneration microenvironment for dental pulp stem cells (DPSCs).
  • To evaluate the biocompatibility of NPM and the effects of NPM-loaded CM on DPSC differentiation and angiogenesis.
  • To assess pulp-like tissue regeneration in vivo.

Main Methods:

  • Developed NPM loaded with CM for DPSCs.
  • Evaluated NPM biocompatibility and the effects of NPM-loaded CM on DPSC differentiation and angiogenesis.
  • Utilized a semi-isotopic in vivo model to assess pulp-like tissue regeneration.

Main Results:

  • NPM demonstrated good biocompatibility.
  • NPM-loaded CM significantly enhanced odontogenic differentiation and angiogenic potential of DPSCs.
  • DPSC + NPM + CM complexes promoted pulp-like tissue regeneration in vivo.

Conclusions:

  • The 3D pulp-specific microenvironment created by NPM and CM bioactive factors facilitates pulp-dentin complex regeneration.
  • This approach offers a novel strategy and experimental basis for endodontic regenerative therapy.