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Updated: Sep 13, 2025

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
Published on: April 12, 2019
KIAA1429-mediated ZFPM2-AS1 m6A modification promotes the proliferation, migration and invasion of osteosarcoma cells
Yuanzhuang Zhang1, Yuxin Bao1, Hanjie Zhai1
1Fourth Department of Orthopedic Surgery, Central Hospital Affiliated to Shenyang Medical College, Shenyang, Liaoning 110024, P.R. China.
Abstract:
Osteosarcoma is the most prevalent malignant bone tumor in pediatric and adolescent populations. N6-methyladenosine (m6A) is a post-transcriptional modification of RNA, and the most prevalent internal chemical modification of mRNA. KIAA1429, also known as virus-like m6A methyltransferase-associated, is a key component of the m6A methyltransferase complex, and the largest protein within this complex. Long non-coding RNAs (lncRNAs) are a class of transcripts >200 nucleotides in length that are able to regulate gene expression. Friend of GATA family member 2-antisense 1 (ZFPM2-AS1) is a lncRNA that has been observed to exhibit aberrantly elevated expression in gastric cancer. The present study aimed to explore the expression levels of KIAA1429 and ZFPM2-AS1 in osteosarcoma tissues and 143B and MG63 osteosarcoma cell lines. The results of bioinformatics analysis, reverse transcription-quantitative PCR (qPCR) and western blotting revealed that the expression of KIAA1429 and ZFPM2-AS1 in osteosarcoma tissues and cell lines was upregulated compared with that in the corresponding normal tissues or cells. The association between KIAA1429-mediated m6A modifications and ZFPM2-AS1 stability in osteosarcoma cells was confirmed using m6A-methylated RNA immunoprecipitation-qPCR and actinomycin D assays. In addition, in Cell Counting Kit-8 and Transwell assays KIAA1429 overexpression promoted the proliferation, invasion and migration of 143B and MG63 osteosarcoma cells, and these promotive effects were attenuated by ZFPM2-AS1 knockdown. These findings suggest a potential novel approach for molecular therapy in the treatment of osteosarcoma.
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