Related Experiment Video

Updated: Sep 13, 2025

Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels
05:31

Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels

Published on: August 7, 2017

10.5K

Mannose-binding lectin insufficiency is associated with airway Haemophilus colonization and a higher risk of post-RSV

Xiaoyan Zhang1, Xiang Zhang1, Xinglan Wang2

  • 1Department of Respiratory Medicine, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, China National Clinical Research Center of Respiratory Disease, Beijing, China.

Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology
|August 1, 2025
PubMed
Abstract

Insights

Mannose-binding lectin (MBL) insufficiency in infants with severe respiratory syncytial virus (RSV) bronchiolitis is linked to recurrent wheezing. MBL insufficiency is associated with Haemophilus colonization and weaker airway interferon-gamma (IFN-γ) response.

Area of Science:

  • Immunology
  • Pediatric Respiratory Medicine
  • Microbiome Research

Background:

  • Severe respiratory syncytial virus (RSV) bronchiolitis in infants can lead to long-term respiratory issues.
  • Mannose-binding lectin (MBL) plays a crucial role in the innate immune system.
  • MBL insufficiency may impact host defense against respiratory infections and subsequent airway inflammation.

Purpose of the Study:

  • To determine if MBL insufficiency affects airway microbiota composition in infants with severe RSV bronchiolitis.
  • To investigate the association between MBL insufficiency and the development of recurrent wheezing in these infants.
  • To explore the relationship between MBL levels, airway microbiota, and immune responses.

Main Methods:

  • Study included 67 infants hospitalized with severe RSV bronchiolitis at <=6 months of age, followed until 3 years.
  • Serum MBL concentrations were measured using ELISA.
  • Sputum microbiota and cytokines were analyzed via 16S rRNA sequencing and multiplex immunoassay.

Main Results:

  • Infants with MBL insufficiency had a significantly higher rate of recurrent wheezing (50.0% vs. 24.4%, p=0.031).
  • MBL insufficiency was independently associated with increased risk of recurrent wheezing (aOR: 3.5, p=0.035).
  • Haemophilus was the most discriminative bacterial genus; lower INF-γ levels were observed in infants with MBL insufficiency.

Conclusions:

  • MBL insufficiency during the first RSV bronchiolitis episode increases the risk of recurrent wheezing by age three.
  • Infants with MBL insufficiency show altered airway microbiota, particularly increased Haemophilus colonization.
  • Weaker airway IFN-γ response is characteristic of MBL insufficiency in this cohort.
Keywords:
IFN‐γMannose‐binding lectininfantsrecurrent wheezingrespiratory microbiomerespiratory syncytial virus

More Related Videos

Absorption of Nasal and Bronchial Fluids: Precision Sampling of the Human Respiratory Mucosa and Laboratory Processing of Samples11:54

Absorption of Nasal and Bronchial Fluids: Precision Sampling of the Human Respiratory Mucosa and Laboratory Processing of Samples

Published on: January 21, 2018

30.9K
Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation08:44

Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation

Published on: May 30, 2020

6.2K

Related Concept Videos

Asthma-II: Pathophysiology and Classification01:26

Asthma-II: Pathophysiology and Classification

Asthma is a prevalent chronic respiratory condition marked by inflammation and hyperresponsiveness of the airways. Its pathophysiology involves complex interactions among inflammatory pathways, immune responses, and neural mechanisms.
Additionally, environmental and genetic factors play crucial roles in determining an individual's susceptibility to asthma and the severity of their condition.
Critical processes in asthma pathophysiology include:
2.9K
Asthma: Pathogenesis and Management01:20

Asthma: Pathogenesis and Management

Asthma is a chronic pulmonary condition involving inflammation of the airways, hyper-reactivity, and reversible obstruction of the airways. This condition can significantly impact a person's quality of life, making breathing difficult and leading to distressing symptoms.
Asthma is classified as allergic and non-allergic. Allergens such as dust mites, pollen, and pet dander trigger allergic asthma, while factors like cold air, intense emotions, or exercise can induce non-allergic asthma.
597
Asthma-I: Introduction01:29

Asthma-I: Introduction

Asthma is a chronic respiratory ailment that requires careful management due to its varying symptoms and influencing factors. It is characterized by airway inflammation, bronchial hyperresponsiveness, and reversible airflow obstruction, leading to symptoms like wheezing, shortness of breath, chest tightness, and coughing. The symptom frequency and intensity may vary considerably over time. It is also linked to immune system responses to allergens and irritants, highlighting the complex...
2.8K
Asthma-III: Symptoms and Complications01:24

Asthma-III: Symptoms and Complications

Asthma, a common chronic respiratory condition, is classified considering the frequency and severity of symptoms alongside lung function impairment. Understanding this classification is essential for appropriate treatment and management. Here's a detailed look at the classification of asthma and its clinical features and complications:
Classification of Asthma
2.6K
Breathing01:05

Breathing

The process of breathing, inhaling and exhaling, involves the coordinated movement of the chest wall, the lungs, and the muscles that move them. Two muscle groups with important roles in breathing are the diaphragm, located directly below the lungs, and the intercostal muscles, which lie between the ribs. When the diaphragm contracts, it moves downward, increasing the volume of the thoracic cavity and creating more room for the lungs to expand. When the intercostal muscles contract, the ribs...
60.3K
Rheumatic Heart Disease I: Introduction01:23

Rheumatic Heart Disease I: Introduction

Rheumatic heart disease or RHD is a chronic condition that results from rheumatic fever, causing permanent damage to the heart valves.Etiology and Risk FactorsIt primarily arises from rheumatic fever, an inflammatory disease that can develop after untreated or inadequately treated group A streptococcal (GAS) pharyngitis. Streptococcus spreads through direct contact with oral or respiratory secretions. While the bacteria are the causative agents, factors like malnutrition, overcrowding, poor...
43