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Updated: Sep 13, 2025
Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells
Published on: February 8, 2018
Association of PD-L1 Expression With Tertiary Lymphoid Structures and Prognosis in Oral Cancer
Emi Saitou1,2, Mai Seki1,2, Takaaki Sano1
1Department of Diagnostic Pathology, GunmaUniversity Graduate School of Medicine, Maebashi, Gunma, Japan.
Background:
Tertiary lymphoid structures (TLSs) are observed within invasive regions of malignancies. This study aimed to elucidate the relationship between programmed death-ligand 1 (PD-L1) expression and TLSs in oral squamous cell carcinoma (OSCC) and to assess their prognostic significance.
Methods:
A total of 280 OSCC cases were analyzed. PD-L1 expression was assessed via immunohistochemistry using biopsy and surgical specimens. Staining for immune cells was performed in cases exhibiting TLSs.
Results:
Patients with high PD-L1 expression and the presence of TLSs demonstrated significantly improved overall survival (p < 0.05). In contrast, in cases with low PD-L1 expression, the presence of TLSs was associated with significantly poorer overall survival (p < 0.05).
Conclusion:
OSCC cases with TLSs, high PD-L1 expression is associated with a favorable prognosis, whereas low PD-L1 expression correlates with significantly worse outcomes. This suggests that TLSs may be functionally heterogeneous, with distinct prognostic implications depending on PD-L1 expression.
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