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Author Spotlight: Unraveling the Interplay Between Trichoderma stromaticum and the Mammalian Immune System
Published on: October 20, 2023
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Novel peptaibiotics identified from Trichoderma clade Viride.
Tamás Marik1, Bonaya Gufu2, Anusha Vishwanathula2
1Department of Biotechnology and Microbiology, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52, 6726, Szeged, Hungary. marik.tamas@szte.hu.
Natural Products and Bioprospecting
|August 1, 2025
Summary
This study identifies novel peptaibiotics, including peptaibols and lipopeptaibols, from Trichoderma fungi. Molecular simulations reveal these compounds tend to form kinked, helical structures in water.
Area of Science:
- Mycology and Natural Products Chemistry
- Biochemistry and Structural Biology
Background:
- Trichoderma fungi are known producers of bioactive secondary metabolites, including peptaibiotics.
- The peptaibiome, encompassing all peptaibiotics produced by an organism, is diverse and incompletely characterized in many fungal species.
Purpose of the Study:
- To investigate and characterize the peptaibiome of various Trichoderma species within the Viride clade.
- To discover and elucidate novel peptaibiotics and their structural properties.
Main Methods:
- High-performance liquid chromatography-mass spectrometry (HPLC-MS) for identification and characterization of peptaibiotics.
- Accelerated molecular dynamics simulations (aMD) to study the folding dynamics and three-dimensional structures of selected peptaibols.
Main Results:
- Identification of 367 peptaibiotics, including 216 peptaibols and 111 lipopeptaibols, with 161 new peptaibols and 73 new lipopeptaibols.
- Introduction of new peptaibol subgroups: Strigosellin A, B and Dorothopsin A, B, and lipopeptaibol sequences: Lipostrigosellins and Lipohamatins.
- Discovery of 41 peptaibol-like compounds with unusual C-termini and observation of a propensity for kinked, right-handed helical structures in peptaibols.
Conclusions:
- The Trichoderma Viride clade harbors a rich and diverse peptaibiome, yielding numerous novel peptaibiotics.
- Structural analysis of Trikoningin KA V suggests specific conformational preferences for Viride clade peptaibols in aqueous environments.
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