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Updated: Jul 10, 2026

ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection
Published on: October 23, 2009
Sensing strips for on-site determination of ochratoxin A in milk and wine
Lavinia Maria Popa-Cobianu1, Raluca-Ioana Stefan-van Staden1, Damaris-Cristina Gheorghe2
1Laboratory of Electrochemistry and PATLAB Bucharest, National Institute of Research for Electrochemistry and Condensed Matter, Bucharest 060021, Romania; Faculty of Chemical Engineering and Biotechnologies, National University of Science and Technology POLITEHNICA of Bucharest, Romania.
Abstract:
Ochratoxin A became a public health concern being one of the mycotoxins that contributes to cancer development. Biomonitoring of ochratoxin A is very important. Therefore, an on-site monitoring of ochratoxin A is proposed based on sensing strips. The sensing strips are stochastic sensors made by deposition of a carbon‑copper nanocomposite layer on silk using cold plasma, and modified with N-(2-mercapto-1H-benzo[d]imidazole-5-yl) oleamide; combined stochastic sensor also has Pt and Ag/AgCl depositions as auxiliary and reference electrodes. Inserted in a portable device, the sensing strips can determine ochratoxin A with high sensitivity (4.29 × 109 s-1 g-1mL), and on a wide working concentration range (1.00 × 10-18-1.00 × 10-3 g mL-1). Recoveries higher than 98.00 % with RSD lower than 1.00 % were recorded when applied for on-site determination of ochratoxin A in wine and milk. The sensing strips will help with fast on-site determination of ochratoxin A from milk and wine in supermarkets, preventing the intoxication of population, and contributing to food security.
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