Related Experiment Video

Updated: Sep 13, 2025

In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter
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In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter

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Regulation, effects and monitoring of compartmented cGMP signalling in cardiomyocytes-current status

Kjetil Wessel Andressen1, Daiana Sedneva-Lugovets1, Mette Ovesen1

  • 1Department of Pharmacology, Institute of Clinical Medicine, University of Oslo and Oslo University Hospital, Oslo, Norway.

British Journal of Pharmacology
|August 2, 2025
PubMed

Insights

Compartmented cyclic guanosine 3

Area of Science:

  • Cardiovascular Biology
  • Cellular Signalling
  • Molecular Cardiology

Background:

  • Cyclic guanosine 3',5'-monophosphate (cGMP) signalling is compartmentalized in cardiomyocytes.
  • This compartmentalization influences cardiac physiology and pathophysiology.

Purpose of the Study:

  • To review the regulation and monitoring of compartmented cGMP signalling in cardiomyocytes.
  • To highlight recent advancements in methodologies for studying cGMP dynamics.

Main Methods:

  • Utilized fluorescence resonance energy transfer (FRET)-based biosensors for visualizing cGMP dynamics.
  • Emphasized the role of guanylyl cyclases (GCs) and phosphodiesterases (PDEs) in regulating cGMP pools.
  • Focused on subcellular localization of GCs and PDEs in defining signalling compartments.

Main Results:

  • Distinct cGMP pools are produced by particulate (GC-A, GC-B) and soluble (sGC) guanylyl cyclases.
  • Phosphodiesterases (PDEs) maintain intracellular cGMP gradients.
  • FRET biosensors revealed localized cGMP signals and discrete signalling compartments.

Conclusions:

  • Understanding compartmented cGMP signalling is crucial for treating cardiac dysfunction.
  • New tools are improving the visualization of cGMP dynamics.
  • This research may lead to novel therapeutic strategies for cardiovascular diseases.

Keywords:
ANPBNPCNPNPR‐ANPR‐Bcardiac myocytescompartmentalized signallingcyclic GMPnatriuretic peptide receptor

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