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Updated: Sep 13, 2025
Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
Published on: November 29, 2024
Identification of mitochondria-related biomarkers for acute respiratory distress syndrome
Huang Hongyuan1,2, Chen Mengchi3, Liang Yingying1
1The Second Affiliated Hospital of Guangxi Medical University, Nanning, 530000, Guangxi, China.
Abstract:
Mitochondria-related genes (MRGs) are considered screening MRGs as biomarkers for important therapeutic targets, but their role in acute respiratory distress syndrome (ARDS) remains unclear. This study aimed to identify MRGs-related biomarkers for ARDS. Intersection of 2030 MRGs with 343 differentially expressed genes (DEGs) between sepsis-non-ARDS and sepsis-ARDS group yielded 20 MRGs-related DEGs enriched in mitochondrion-related pathways. Monocytes, T cells, natural killer (NK) T cells, NK cells, and innate lymphoid cells (ILCs) were screened as candidate key cell types. SLC2A1 and IFI27 were defined as biomarkers; naive B cells, resting NK cells, and naive CD4 T cells, as differential immune cells; and monocytes, as the key cell type, subdivided into the CD14 and CD16 subtypes. The sepsis-ARDS group exhibited enhanced CD14 Mono and B cells compared with the sepsis-non-ARDS group and CD16 had a higher percentage and score in the G2/M and S phases than CD14. IFI27 and SLC2A1 were mainly expressed differently in the CD14 monocytes in ARDS, and their mRNA expression levels in peripheral blood of the sepsis patients was upregulated compared with those of the healthy controls. Thus, our findings demonstrate that SLC2A1 and IFI27 represent novel MRGs-related diagnostic biomarkers for ARDS, providing theoretical references for its treatment.
Insights
Mitochondria-related genes (MRGs) show promise as biomarkers for acute respiratory distress syndrome (ARDS). SLC2A1 and IFI27 are identified as novel diagnostic biomarkers for ARDS, aiding in treatment strategies.
Area of Science:
- Molecular biology
- Immunology
- Genetics
Background:
- Mitochondria-related genes (MRGs) are crucial for cellular functions and disease processes.
- The specific role of MRGs in acute respiratory distress syndrome (ARDS) requires further elucidation.
- Identifying reliable biomarkers is essential for early diagnosis and effective treatment of ARDS.
Purpose of the Study:
- To identify novel mitochondria-related gene (MRG)-based biomarkers for acute respiratory distress syndrome (ARDS).
- To investigate the role of specific immune cell types in ARDS pathogenesis.
- To explore the diagnostic potential of identified biomarkers in sepsis-induced ARDS.
Main Methods:
- Differential gene expression analysis comparing sepsis-ARDS and sepsis-non-ARDS groups.
- Intersection of mitochondria-related genes (MRGs) with differentially expressed genes (DEGs).
- Bioinformatic analysis to identify key cell types and biomarkers, including immune cell profiling.
Main Results:
- Twenty MRG-related DEGs were identified, enriched in mitochondrion-related pathways.
- SLC2A1 and IFI27 were identified as potential diagnostic biomarkers for ARDS.
- Monocytes, particularly CD14+ subtypes, and B cells showed differential expression in sepsis-ARDS patients. IFI27 and SLC2A1 were upregulated in CD14 monocytes of ARDS patients.
Conclusions:
- SLC2A1 and IFI27 are novel, promising MRG-related diagnostic biomarkers for ARDS.
- These findings offer a theoretical basis for developing new therapeutic strategies for ARDS.
- The study highlights the importance of immune cell dynamics, especially monocytes, in ARDS pathophysiology.
