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Updated: Sep 13, 2025
12:57
Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
Published on: December 21, 2017
11.6K
Ribosome profiling sequencing reveals translational dynamics during yak testicular development
Shaoke Guo1, Mengli Cao1, Xingdong Wang1
1Key Laboratory of Yak Breeding in Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China; Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou 730050, China.
International Journal of Biological Macromolecules
|August 3, 2025
Summary
This study reveals dynamic translation regulation during yak testicular development. Key genes and pathways are identified, offering insights to improve yak reproductive performance.
Area of Science:
- Reproductive Biology
- Molecular Biology
- Genomics
Background:
- Translation regulation is vital for testicular development and spermatogenesis.
- The dynamic mechanisms governing these processes remain unclear.
Purpose of the Study:
- To investigate the translation landscape of yak testes across different developmental stages.
- To elucidate the dynamic regulation of gene translation during yak sexual maturation.
Main Methods:
- Integrated transcriptome and ribosomal sequencing (Ribo-seq) data from yak testes at 6 months, 18 months, and 4 years.
- Analyzed ribosome footprint characteristics, translation efficiency (TE), and protein-protein interactions (PPI).
- Identified differentially translated genes, stage-specific regulatory genes, and translatable small open reading frames (sORFs).
Main Results:
- Yak ribosome footprint characteristics align with other mammals.
- Differentially translated genes are enriched in meiotic cell cycle, PI3K Akt, and Notch signaling pathways.
- Identified stage-specific genes (COL1A2/MEIOB/SYCP3 and STAT1/ITGB5/ERBB2) and 106 predicted translatable sORFs.
- Translation efficiency correlates with uORF length, GC content, CDS length, and NMEF.
Conclusions:
- Provides novel insights into dynamic gene translation regulation during yak testicular development and spermatogenesis.
- Highlights the significance of these findings for enhancing yak reproductive performance.
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