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Updated: Sep 13, 2025
In Vitro ELISA Test to Evaluate Rabies Vaccine Potency
Published on: May 11, 2020
Development of a competitive ELISA using monoclonal antibodies for detecting neutralizing antibodies against
Linjie Hu1, Haoyue Xu1, Xueliang Zhu1
1State Key Laboratory for Animal Disease Control and Prevention/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Abstract:
Peste-des-petits-ruminants (PPR) is an acute, contact infectious disease caused by peste-des-petits-ruminants virus (PPRV) infection. PPRV mainly infects goats, sheep and other small ruminants, posing a serious threat to the development of the livestock industry and the safety of wildlife. In this study, a competitive ELISA (c-ELISA) method for detecting neutralizing antibodies against PPRV was developed using the extracellular domain of haemagglutinin (tH) protein and a monoclonal antibody against tH protein. The results showed that the specificity and sensitivity of c-ELISA were 99.38% and 100%, respectively. The agreement rate of the c-ELISA and viral neutralization test (VNT) was 96.65%, suggesting that the c-ELISA could detect neutralizing antibody against PPRV and could be used for protective immunity evaluation.
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