A Rapid and Simple Reversed-Phase HPLC-MS/MS Determination of Baloxavir in Rat Plasma and Its Application in
Peng Guoshuang1, Wan Lili2, Liu Xiang3
1Division of Pharmaceutics, National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang, China.
Abstract:
In this study, a novel RP-HPLC-MS/MS method was developed for the accurate quantification of baloxavir in rat plasma. The analytes were retained and separated on a C18 column, and a triple-quadrupole mass spectrometer equipped with an electrospray ionization (ESI) source was applied for quantification. Baloxavir could be well retained in the C18 column under the appropriate chromatographic conditions. Using a simple protein precipitation method, the extraction recovery was about 100%, and the ME could be negligible. The method demonstrated excellent linearity (r = 0.9995) across the concentration range of 1.59 ~ 3180.00 ng/mL for baloxavir. The precision and accuracy were within acceptable limits. The lowest limit of quantification (LLOQ) was determined to be 1.59 ng/mL. This simple method was validated and successfully applied to support the pharmacokinetics study after baloxavir was administered to the Sprague-Dawley rats.
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