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Phytochemical Characterization of Pouteria guianensis: Optimized Flavonoid Extraction and HPLC Method Validation
Rafael Christian de Matos1,2, Ana Flávia Alvarenga Bitencourt1, Alexsandro Davi Mantovani de Oliveira1
1Faculdade de Farmácia, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, Campus Pampulha, 31.270-901 Belo Horizonte, MG, Brazil.
ACS Omega
|August 4, 2025
Summary
This study optimized flavonoid extraction from Pouteria leaves, identifying myricitrin as a key compound. The findings pave the way for developing new phytotherapeutics from Brazilian plants.
Area of Science:
- Pharmacognosy and Phytochemistry
- Natural Product Chemistry
- Analytical Chemistry
Background:
- Brazil's plant biodiversity holds potential for phytotherapeutic development, particularly the Pouteria genus.
- Pouteria species are traditionally used but scientifically underexplored for their medicinal properties.
- Flavonoids are a significant class of compounds with therapeutic potential.
Purpose of the Study:
- To optimize flavonoid extraction from Pouteria leaves using response surface methodology.
- To develop and validate a high-performance liquid chromatography (HPLC) method for flavonoid analysis.
- To characterize a chemical marker in Pouteria species for future phytotherapeutic applications.
Main Methods:
- Response surface methodology was employed to optimize extraction parameters: time, drug-to-solvent ratio, temperature, and cycles.
- A high-performance liquid chromatography (HPLC) method was developed and validated.
- Myricitrin was used as the analytical marker, with quercetin as the external standard for quantification.
Main Results:
- Optimized extraction conditions: 2 cycles, 8 min each, at 21°C, with a 1:8 drug-to-solvent ratio.
- The HPLC method demonstrated effective validation for quantitative analysis.
- Myricitrin content was determined to be 2.96 mg/g (0.30%) in the Pouteria extract.
Conclusions:
- The study successfully optimized flavonoid extraction from Pouteria leaves.
- A validated HPLC method provides a reliable tool for chemical marker analysis.
- These findings support the potential of Pouteria species in phytotherapeutic development and offer a method applicable to related species.
