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Related Experiment Video

Updated: Sep 12, 2025

Normothermic Ex Vivo Liver Machine Perfusion in Mouse
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An Open-Source Normothermic Perfusion System Designed for Research Scientists.

Lindsay R Friedman1, Hannah E Stepp1, Jacob Lambdin1

  • 1Surgical Oncology Program, National Cancer Institute, National Institutes of Health.

Journal of Visualized Experiments : Jove
|August 4, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed a low-cost, modular normothermic perfusion machine using accessible parts and Python code. This system enhances organ research accessibility and enables detailed study of cellular processes in intact human organs.

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Last Updated: Sep 12, 2025

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Area of Science:

  • Biomedical Engineering
  • Translational Medicine
  • Organ Transplantation

Background:

  • Ex vivo perfusion machines improve organ transplantation outcomes and availability.
  • Current machines are costly and lack experimental flexibility for researchers.
  • There is a need for accessible pre-clinical models for studying cellular processes in whole organs.

Purpose of the Study:

  • To develop a cost-effective, modular normothermic perfusion machine for broader research access.
  • To create a customizable system using readily available components and open-source code.
  • To enable detailed investigation of cellular processes in intact organs.

Main Methods:

  • Construction of a modular normothermic perfusion machine from standard parts.
  • Utilization of user-modifiable Python code for system control.
  • Testing with porcine and human organs, detailing setup and perfusion protocols.

Main Results:

  • Successful construction and operation of a cost-effective perfusion system.
  • Demonstrated feasibility with both porcine and human organs.
  • Early proof-of-concept experiments showcasing interrogation of pathophysiology in intact tissues.

Conclusions:

  • The developed perfusion machine provides an accessible platform for organ research.
  • The modular design allows for customization for diverse research needs.
  • This system facilitates the study of complex cellular processes and pathophysiology in human organs.