Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Upstream Processing01:27

Upstream Processing

Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ExIR enables prioritizing driver and biomarker genes from omics data in a reference free manner.

iScience·2026
Same author

Application of spatial transcriptomics across organoids for a high-resolution, spatial whole-transcriptome benchmarking dataset.

iScience·2026
Same author

Discordance Between Systemic Lupus Erythematosus Disease Activity Index Domain Weights and Their Association With Organ Damage Accrual.

Arthritis care & research·2026
Same author

Macrophages orchestrate antiviral defense and epithelial repair in a human iPSC-derived alveolar air-liquid interface.

JCI insight·2026
Same author

The emergence of multiple testicular cell lineages in human stem cell-derived testis-like organoids.

Development (Cambridge, England)·2026
Same author

Transcriptomic signatures in brain and blood related to cognitive and psychiatric phenotypes of Prader-Willi syndrome.

Scientific reports·2025
Same journal

A Video Protocol of a Randomized Controlled Clinical Trial - Electrochemotherapy of Cutaneous Metastases with Reduced Dose Bleomycin (BLESS Trial).

Journal of visualized experiments : JoVE·2026
Same journal

A Standardized Ex Vivo Porcine Oromucosal Model for Evaluating Peptide Fluxes.

Journal of visualized experiments : JoVE·2026
Same journal

Lightweight English Text Classification with Deep Learning Based on Complex System Theory.

Journal of visualized experiments : JoVE·2026
Same journal

Integrating Artificial Intelligence-Assisted Translation Support into English Courses: Effects on Translation Accuracy, Perceived Stress, and Anxiety.

Journal of visualized experiments : JoVE·2026
Same journal

A Toxin-Based Counter-Selection System for Markerless Gene Deletion and High-Density Tn5 Transposon Mutagenesis in Pectobacterium brasiliense.

Journal of visualized experiments : JoVE·2026
Same journal

Seamless Multimodal Human-Robot Communication: Integration Techniques in Human-Computer Interaction.

Journal of visualized experiments : JoVE·2026
See all related articles

Related Experiment Video

Updated: May 25, 2026

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
09:07

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Published on: June 21, 2016

8.3K

A Web-Based Workflow for Selecting Gene- and Tissue-Specific Enhancers.

Jooa Kwon1, George Z He2, Mirana Ramialison3

  • 1Department of Paediatrics, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne; Australian Regenerative Medicine Institute, Monash University.

Journal of Visualized Experiments : Jove
|August 4, 2025
PubMed
Summary
This summary is machine-generated.

Scientists developed a user-friendly, web-based protocol to identify gene enhancers. This tool helps biologists discover enhancers regulating specific genes, aiding disease research.

More Related Videos

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
10:46

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

Published on: June 2, 2018

9.4K
AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain
09:59

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain

Published on: March 31, 2022

2.8K

Related Experiment Videos

Last Updated: May 25, 2026

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
09:07

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Published on: June 21, 2016

8.3K
Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
10:46

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

Published on: June 2, 2018

9.4K
AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain
09:59

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain

Published on: March 31, 2022

2.8K

Area of Science:

  • Genomics
  • Molecular Biology
  • Epigenetics

Background:

  • Enhancers are crucial DNA regions regulating gene expression, but their identification is challenging as they lack protein-coding sequences.
  • Mutations in enhancers can disrupt gene regulation, contributing to various diseases.
  • Existing enhancer identification tools are often complex, posing a barrier for biologists.

Purpose of the Study:

  • To present a biologist-friendly, web-based protocol for identifying enhancers associated with a specific gene of interest (GoI).
  • To simplify the process of enhancer discovery for researchers without programming expertise.
  • To leverage existing genomics data for enhancer identification in a target tissue.

Main Methods:

  • The protocol utilizes web-based genomics data, including H3K4me1 and H3K27ac histone modifications and chromatin conformation capture (Hi-C) data.
  • It enables the discovery of enhancers linked to a gene of interest (GoI) within a specific, active tissue.
  • The workflow is entirely web-based, requiring no specialized programming skills.

Main Results:

  • The protocol successfully identified candidate enhancers regulating TBX5, a gene vital for heart development.
  • The study demonstrated the protocol's utility in characterizing enhancers active in the left ventricle.
  • The approach facilitates the association of enhancers with specific genes in relevant biological contexts.

Conclusions:

  • The developed protocol offers a simplified, accessible method for enhancer identification.
  • This tool empowers biologists to explore gene regulatory elements and their role in development and disease.
  • The protocol enhances the understanding of gene-tissue specific regulation by identifying relevant enhancers.