JoVE - Journal of Visualized Experiments
JoVE Visualize
Contact Us

Prognostic significance of APOBEC1 and its role in lung squamous cell carcinoma: insights from chromatin regulator-based modeling and experimental validation

  • 0Jinan University, Guangzhou, China.
Expert Review of Anticancer Therapy +

|

August 5, 2025

|

August 5, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

This study developed a prognostic model using eight chromatin regulators (CRs) for lung squamous cell carcinoma (LUSC). The model predicts patient survival and identifies APOBEC1 as a potential therapeutic target in LUSC.

Area Of Science

  • Oncology
  • Molecular Biology
  • Genomics

Background

  • Chromatin regulators (CRs) play a role in cancer development.
  • The prognostic significance of CRs in lung squamous cell carcinoma (LUSC) is not well understood.
  • APOBEC1's role in LUSC progression requires further investigation.

Purpose Of The Study

  • To develop a prognostic model for LUSC based on chromatin regulators.
  • To explore the prognostic value of these regulators in LUSC.
  • To investigate the role of APOBEC1 in LUSC progression and its potential as a therapeutic target.

Main Methods

  • Analysis of transcriptomic and clinical data from TCGA and GEO databases.
  • Identification of prognostic CRs using univariate Cox and Lasso regression analyses.
  • Experimental validation of APOBEC1 function in LUSC cell lines using in vitro assays.

Main Results

  • An eight-gene CR-based signature demonstrated strong prognostic performance across multiple datasets (5-year AUCs 0.73-0.92).
  • High-risk patients exhibited enrichment in cancer pathways, increased immune infiltration, and sensitivity to specific drugs (Dasatinib, Bexarotene, Bicalutamide).
  • APOBEC1 was found to be overexpressed in LUSC and promoted cancer cell proliferation and migration.

Conclusions

  • A robust CR-based prognostic model for LUSC survival was established.
  • APOBEC1 is identified as a potential therapeutic target for LUSC.
  • This research provides insights into the molecular mechanisms of LUSC and potential therapeutic strategies.

Keywords: