Hippocampal architecture viewed through the eyes of methodological development

This review focuses on the structural organization of the hippocampus and how our understanding of its cellular architecture and functional circuits has been enabled over the last 400 years by the development of methods as varied as the Golgi impregnation, immunohistochemical staining procedures, and polarized light imaging. We provide an outlook on how cutting-edge techniques such as high-resolution imaging and artificial intelligence may continue to shed light on the structural organization of the hippocampus and emphasize the importance of collaborative multidisciplinary efforts including classical neuroanatomists in this endeavor.