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Related Experiment Video

Updated: Sep 12, 2025

A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice
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Live Cell Imaging for Keratinocyte Lineage Tracing.

Brook Abegaze1,2, Brooke Vittimberga1, Nwamaka Ijeh1

  • 1The University of California, San Francisco, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 5, 2025
PubMed
Summary
This summary is machine-generated.

This study details a method for live cell imaging of human keratinocytes to analyze cell division kinetics. The technique allows for precise measurement of cell cycle duration and division fates in vitro.

Keywords:
Cell cycleDivision fateDivision kineticsKeratinocytesLineage tracingLive cell imagingProliferationTime lapse photography

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Area of Science:

  • Cell Biology
  • Dermatology
  • Microscopy

Background:

  • Live cell imaging is crucial for studying cellular processes.
  • Understanding keratinocyte division is vital for skin biology and wound healing.
  • Standard methods may not capture single-cell dynamics accurately.

Purpose of the Study:

  • To present a standardized method for live cell imaging of human keratinocytes.
  • To enable detailed analysis of keratinocyte division kinetics and cell fates.
  • To provide a framework for assessing proliferation and differentiation.

Main Methods:

  • Culture of unpassaged human keratinocytes at clonal density.
  • Time-lapse photography to record single-cell divisions.
  • Construction of lineage trees for kinetic analysis.

Main Results:

  • The described method allows for accurate determination of cell cycle duration.
  • Individual keratinocyte division fates (proliferation vs. differentiation) can be assessed.
  • The technique minimizes confounding factors from the culture environment.

Conclusions:

  • This method provides a robust approach to study human keratinocyte behavior in vitro.
  • It facilitates detailed insights into cell cycle dynamics and fate decisions.
  • The technique is valuable for research in skin biology and regenerative medicine.