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Comprehensive evaluation of Xuezhikang by four-dimensional fingerprint combined with antioxidant
Bozhi Yang1, Xinrong Liu1, Xiangfei Sun1
1Shenyang Pharmaceutical University, Shenyang 110016, China.
Insights
This study evaluated the quality of Xuezhikang (XZK) capsules using advanced analytical techniques. The five-wavelength fusion fingerprint (FWFFT) method, combined with other analyses, established a grading system for XZK quality and demonstrated its antioxidant properties.
Area of Science:
- Pharmaceutical Analysis
- Traditional Chinese Medicine Quality Control
- Spectroscopy
Background:
- Xuezhikang (XZK) is reported to modulate blood lipid levels, reducing cardiovascular risks in hypertensive and dyslipidemic patients.
- Ensuring the consistent quality of XZK is crucial for its therapeutic efficacy and patient safety.
Purpose of the Study:
- To investigate the quality consistency of XZK capsules.
- To establish a comprehensive quality control strategy for XZK using multi-technique analysis.
- To explore the spectrum-effect relationship between XZK's chemical fingerprint and its antioxidant activity.
Main Methods:
- Utilized a five-wavelength fusion fingerprint (FWFFT) method combined with UV, FT-IR, and DSC analysis.
- Applied the systematically quantified fingerprint method (SQFM) for multi-wavelength fusion of HPLC fingerprints.
- Conducted in vitro antioxidant activity assays and analyzed the spectrum-effect relationship using grey correlation analysis.
Main Results:
- The four-dimensional fingerprint classified XZK samples into four distinct grades, indicating variations in quality.
- Identified significant antioxidant activity in the active components lovastatin and genistein within XZK.
- Demonstrated that FWFFT technology effectively compensates for single-wavelength limitations and reduces bias from single-technology evaluations.
Conclusions:
- The integrated FWFFT method, alongside UV, FT-IR, and DSC, provides a robust and reliable approach for comprehensive quality control of XZK.
- This study offers a new direction for integrating pharmaceutical analysis technologies for traditional Chinese medicine quality evaluation.
- The developed methodology ensures a safe, reliable, and convenient way for the comprehensive quality control of Xuezhikang capsules.
Abstract:
It is reported that XZK has the ability to modulate blood lipid levels, thereby decreasing the likelihood of cardiovascular incidents and overall mortality among patients suffering from hypertension and dyslipidemia. To ensure the overall quality of XZK, strict quality control measures are essential. In this study, the quality consistency of XZK capsules was investigated using a five-wavelength fusion fingerprint (FWFFT) method, complemented by all-ultraviolet spectroscopy (UV), Fourier transform infrared spectroscopy (FT-IR) and Differential scanning calorimeter (DSC) analysis. The systematically quantified fingerprint method (SQFM) was applied to merge the five HPLC fingerprint wavelengths of 30 batches of XZK samples into a single representative wavelength using multi-wavelength fusion technology for consistency evaluation. An in vitro antioxidant activity assay was conducted, and the relationship between antioxidant activity and the HPLC fingerprint was analyzed using the grey correlation method to establish a spectrum-effect relationship. The results of four-dimensional fingerprint indicated that the samples were classified into four distinct grades. S9 and S23 were categorized as grade 2, S2 as grade 4, S30 as grade 6, and all other samples as grade 1, and the antioxidant results showed that the two quantitative active components, lovastatin and genistein, in XZK had significant antioxidant activity. This study integrates the variations in different sample characteristics using the four-dimensional fusion fingerprint, and these differences are represented through a grading system. FWFFT technology effectively compensates for the limitations of single-wavelength evaluation methods and, when combined with UV, FT-IR, and DSC, reduces the bias introduced by single-technology evaluations. The quality evaluation of Xuezhikang capsules by using systematic quantitative fingerprinting technology combined with various techniques provides a new direction for the integration of pharmaceutical analysis technologies, it provides a safe, reliable and convenient way for the comprehensive quality control of traditional Chinese medicine.
